Anti-KCNK6 (TWIK-2) Antibody

Potassium channel subfamily K member 6, TOSS, K2P6.1
    Cat #: APC-040
    Alternative Name Potassium channel subfamily K member 6, TOSS, K2P6.1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
      • Peptide (C)ESHQQLSASSHTDYASIPR, corresponding to residues 295-313 of human KCNK6 (Accession Q9Y257). Intracellular, C-terminus.
        Anti-KCNK6 (TWIK-2) Antibody
    Accession (Uniprot) Number Q9Y257
    Gene ID 9424
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 13/17 amino acid residues identical.
    RRID AB_2040142.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-KCNK6 (TWIK-2) Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-KCNK6 (TWIK-2) Antibody (#APC-040), (1:200).
        2. Anti-KCNK6 (TWIK-2) Antibody, preincubated with the control peptide antigen.
      • KCNK6 (also named Tandem of P domain of Weak Inward rectifier K+ channel 2, TWIK-2 or K2P6.1) is a member of the 2-pore (2P) domain K+ channel family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.

        The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. The functional channel is believed to be a dimer.

        K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.

        KCNK6 displays low single channel conductance of about 5pS and is partially activated by arachidonic acid and blocked by volatile anesthetics.

        KCNK6 expression is widespread, especially in the rat were it is expressed in brain, heart, kidney and liver. In humans KCNK6 expression is more restricted with expression in aorta, stomach, placenta and spleen while in mouse, KCNK6 expression has been detected only in liver.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-KCNK6 (TWIK-2) Antibody (#APC-040) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize KCNK6 from rat and human samples.

    For research purposes only, not for human use