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Anti-KCC1 (SLC12A4) Antibody

Erythroid K+-Cl- cotransporter 1, Electroneutral potassium-chloride cotransporter 1

Cat #: AKT-001
Alternative Name Erythroid K+-Cl- cotransporter 1, Electroneutral potassium-chloride cotransporter 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)HAPDNFRELVHIK, corresponding to amino acid residues 998-1010 of rat KCC1 (Accession Q63632). Intracellular, C-terminus.
Accession (Uniprot) Number Q63632
Gene ID 29501
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2340982.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Several splice variants of the KCC1 human gene have been identified. This antibody will recognize all known variants except for isoform 4. For more information go to human KCC1 (Accession Q9UP95).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human K562 erythroleukemia cell lysates (lanes 3 and 6):
    Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human K562 erythroleukemia cell lysates (lanes 3 and 6):
    1-3. Anti-KCC1 (SLC12A4) Antibody (#AKT-001), (1:200).
    4-6. Anti-KCC1 (SLC12A4) Antibody, preincubated with KCC1/SLC12A4 Blocking Peptide (#BLP-KT001).
References
  1. Russell J.M. (2000) Physiol. Rev. 80, 211.
  2. Nagao, H. et al. (2012) Cell. Physiol. Biochem. 30, 538.
  3. Gamba, G. (2005) Physiol. Rev. 85, 423.
  4. Delpire, E. and Gagnon, K.B. (2008) Biochem. J. 409, 321.
  5. Lee, H.H. et al. (2007) J. Biol. Chem. 282, 29777.
  6. Gillen, C.M. et al. (1996) J. Biol. Chem. 271, 16237.
  7. Olivieri, O. et al. (1992) Blood 79, 793.
Scientific background

Potassium-chloride cotransporters (KCCs) are members of the cation chloride cotransporter gene (Slc) superfamily. This family consists of sodium-potassium-two chloride cotransporter 1 (NKCC1) and 2 (NKCC2), sodium-chloride cotransporter (NCC) and KCCs1. All of these transporters mediate electroneutral transport of cations coupled with one or two Cl- ions and contribute to regulation of cell volume and homeostasis of intracellular Cl- concentration. KCCs extrude one K+ with one Cl- from the intracellular space to the extracellular space by using the chemical gradient of K+ as the driving force2.

Four KCC isoforms (KCC1, KCC2, KCC3 and KCC4) have been identified in mammalian species3.

K-Cl cotransporters regulate neuronal and glial electrochemical equilibrium potential for Cl-, and so can determine the excitatory or inhibitory influences of GABA- and glycine-gated Cl- channels4. KCC1 is a member of the cation-chloride cotransporter (CCC) superfamily of proteins, as with all CCC proteins, KCC1 is an integral membrane protein with 12 transmembrane domains and both N- and C-terminal cytoplasmic domains 5. KCC1 is ubiquitously expressed6.

K-Cl cotransport activity is elevated in red blood cells of individuals with sickle cell disease, thalassemia, and diseases associated with other mutant Hb. Human erythrocytes from individuals with the reticulocytotic anemic states of sickle cell disease, Hb SC disease, and β-thalassemia intermedia exhibit uniformly elevated KCC1-like immunoreactivity compared with normal AA erythrocytes7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/02/2022

Alomone Labs is pleased to offer a highly specific antibody directed against epitope of rat KCC1. Anti-KCC1 (SLC12A4) Antibody (#AKT-001) can be used in western blot analysis. It has been designed to recognize KCC1 from rat, mouse and human samples. Several splice variants of the KCC1 human gene have been identified. This antibody will recognize all known variants except for isoform 4. For more information go to human KCC1 (Accession Q9UP95).

For research purposes only, not for human use

Applications

Citations

Citations
Western blot citations
  1. Rat colon sections.
    Tang, L. et al. (2017) PLoS ONE 12, e0171045.

Specifications

Scientific Background

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