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3,4. Guinea pig Anti-GIRK2 (Kir3.2) Antibody, preincubated with the control antigen.
Following a screen of several secondary antibodies, the following were used for western blot analysis:
Anti-Guinea pig IgG (Sigma #A7289 or Jackson ImmunoResearch #106-035-006).
- Hibino, H. et al. (2010) Physiol. Rev. 90, 291.
Inward rectifying K+ (Kir) channels generate under physiological conditions, large K+ conductance at potentials negative to Ek but permit less current flow at potentials positive to Ek.
Kir3.2 (also known as GIRK2 or KCNJ6) belongs to a G-protein gated subfamily of Kir channels. Kir3.2 is one of four subunits (Kir3.1, Kir3.3 and Kir3.4) that form functional tetrameric KG channel assemblies. KG channels are activated by GTP (GTPi) in the presence of an agonist or by intracellular GTPγS in the absence of an agonist. There are at least four different isoforms of Kir3.2 which are generated by alternative splicing from a single gene. Kir3.2 isoforms exhibit differential expression patterns in various tissues such as brain, pituitary, pancreas, and testis, where they usually coincide with the expression of other Kir3.x subunits.
Kir3.2 is expressed on the cell surface due to two forward trafficking signal in its cytoplasmic region. One is an ER export signal in its NH2 terminus and the other is a post-ER trafficking signal that is composed of a cluster of acidic residues in the COOH terminus.
Knockout of Kir3.2 is accompanied by a decrease in Kir3.1 protein expression in the brain, the development of spontaneous seizures, and a range of phenotypes related to sporadic seizures1.
Species reactivity key:
Double staining of GIRK2 channels in substantia nigra pars compacta using Tertiapin-Q-ATTO-488 and Guinea pig Anti-GIRK2 (Kir3.2) Antibody.
Rat brain sections were first incubated with 33 nM Tertiapin-Q-ATTO-488 (#STT-170-AG), (green). The same sections were incubated with Guinea pig Anti-GIRK2 (Kir3.2) Antibody (#AGP-013). A. Tertiapin-Q-ATTO-488 binding appears in profiles of substantia nigra pars compacta (SNC) neurons (arrows). B. GIRK2 staining (red) is detected in profiles of SNC neurons (arrows). C. Merge of the two images demonstrates co-staining of GIRK2 channels by Tertiapin-Q-ATTO-488 and by Guinea pig Anti-GIRK2 (Kir3.2) Antibody. DAPI staining (blue) is used to stain nuclei.
Guinea pig Anti-GIRK2 (Kir3.2) Antibody (#AGP-013) raised in guinea pigs is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GIRK2 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-GIRK2 (Kir3.2) Antibody (#APC-006) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.
Products for control experiments
- GIRK2 (Kir3.2) Channel Overexpressed Membrane Fractions (#LX-102). The GIRK2 overexpressed membrane fractions enables end users to check the expression of endogenous GIRK2 as opposed to a tagged version and serves as a positive control for testing the expression of the channel and a quick way to test the antibody.
- GIRK2 (Kir3.2) Channel Antibody and Membrane Fractions Kit (#LK-102). This kit includes the GIRK2 overexpressed membrane fractions, and Anti-GIRK2 (Kir3.2) Antibody (#APC-006).