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2. Anti-GIRK2 (Kir3.2) Antibody, preincubated with the control antigen.


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Kir3.2 (or G-protein regulated inward-rectifier K+ channel, GIRK2) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.
The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.
Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.
Kir3.2 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Gα-GTP from the Gbg dimer. The latter directly binds to Kir3 and activates the channel.2,3
Kir3.2 is mainly expressed in the brain, where it co-assembles with Kir3.1 (GIRK1) or Kir3.3 (GIRK3) and mediates the inhibitory effects of many neurotransmitters including opioid, adrenergic, muscarinic, dopaminergic and GABAergic neurotransmitters.2,3
Point mutations in the mouse Kir3.2 channel cause the weaver (wv) phenotype, a neurological abnormality characterized by the abnormal ‘weaving’ of the mice when they walk, hence the name weaver which is due to a substantial loss of cerebellar granule neurons. These mice also display mild local motor hyperactivity, presumably caused by the degeneration of dopaminergic neurons in the substantia nigra, spontaneous seizures and male sterility.1
A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.2 containing channels (7 nM for Kir3.2 alone and 5.4 nM for the Kir3.1/3.2 combination).4
Application key:
Species reactivity key:
Expression of GIRK2 in mouse hippocampus.Western blot analysis of mouse hippocampus lysate using Anti-GIRK2 (Kir3.2) Antibody (#APC-006) (upper panels). Note lack of GIRK2 in knockout mice. Immunocytochemical staining of cultured hippocampal mouse neurons with Anti-GIRK2 (Kir3.2) Antibody in wild type cells (upper staining) and Girk2-/- cells (lower staining).Adapted from Marron Fernandez de Velasco, E. et al. (2017) Sci. Rep. 7, 1639. with permission of SPRINGER NATURE.
Anti-GIRK2 (Kir3.2) Antibody (#APC-006) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Kir3.2 from rat, mouse, and human samples.
- Immunohistochemical staining of mouse brain sections (1:400). Also tested in GIRK2 conditional knockout mice.
Honda, I. et al. (2018) Neurosci. Lett. 665, 140. - Western blot analysis of mouse primary hippocampal culture lysate. Immunocytochemical staining of mouse cultured hippocampal neurons. Also tested in Girk2-/- mice.
Marron Fernandez de Velasco, E. et al. (2017) Sci. Rep. 7, 1639.
- Mouse brain lysate.
Tang, X. et al. (2018) Neuroscience 373, 52. - Mouse primary hippocampal culture lysate. Also tested in Girk2-/- mice.
Marron Fernandez de Velasco, E. et al. (2017) Sci. Rep. 7, 1639. - Mouse hippocampus lysate.
May, L.M. et al. (2017) Front. Neurosci. 11, 456. - Mouse perirhinal cortex lysate.
Roncace, V. et al. (2017) Neurobiol. Dis. 106, 89. - Mouse brain lysate.
Joshi, K. et al. (2016) Ann. Neurol. 80, 511. - Mouse brain lysate (1:200).
Kotecki, L. et al. (2015) J. Neurosci. 35, 7131. - Mouse brain lysate (1:1000).
Goodfellow, N.M. et al. (2014) J. Neurosci. 34, 6107. - Mouse brain lysate (1:200).
Hearing, M. et al. (2013) Neuron 80, 159.
- Mouse brain membrane proteins (1:174).
Jelacic, T.M. et al. (2000) J. Biol. Chem. 275, 36211.
- Mouse brain sections (1:400). Also tested in GIRK2 conditional knockout mice.
Honda, I. et al. (2018) Neurosci. Lett. 665, 140. - Monkey brain sections.
Kikuchi, T. et al. (2017) Nature 548, 592. - Rat brain sections.
Niclis, J.C. et al. (2017) Stem Cell Reports 9, 868. - Mouse brain sections (1:100).
Stott, S.R.W. et al. (2017) PLoS ONE 12, e0171748. - Mouse brain sections (1:600).
Brandalise, F. et al. (2016) Eur. J. Neurosci. 43, 1460. - Rat brain sections (1:500).
Montandon, G. et al. (2016) Sci. Rep. 6, 32707. - Rat brain sections.
Seiler, S. et al. (2016) Front. Cell. Neurosci. 10, 87. - Mouse embryo sections.
Bye, C.R. et al. (2015) Proc. Natl. Acad. Sci. U.S.A. 112, E1946. - Mouse brain sections (1:1000).
Kotecki, L. et al. (2015) J. Neurosci. 35, 7131. - Mouse brain sections (1:600).
Bodea, G.O. et al. (2014) Development 141, 661. - Mouse and Rat DRG, sciatic nerve and skin sections (1:200).
Nockemann, D. et al. (2013) EMBO Mol. Med. 5, 1263. - Human skin sections (1:200).
Nockemann, D. et al. (2013) EMBO Mol. Med. 5, 1263. - Mouse spinal cords.
Marker, C.L. et al. (2004) J. Neurosci. 24, 2806.
- Mouse cultured hippocampal neurons. Also tested in Girk2-/- mice.
Marron Fernandez de Velasco, E. et al. (2017) Sci. Rep. 7, 1639. - hVM1 (human ventral mesencephalic neural stem cell line 1 (1:400).
Courtois, E.T. et al. (2010) J. Biol. Chem. 285, 9881.
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- Friling, S. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 7613.
- Kolk, S.M. et al. (2009) J. Neurosci. 29, 12542.
- Iwanir, S. and Reuveny, E. (2008) Pflugers Arch. 456, 1097.
- Lignon, J.M. et al. (2008) Physiol. Genomics 33, 230.
- Labouèbe, G. et al. (2007) Nat. Neurosci. 10, 1559.
- Kulik, A. et al. (2006) J. Neurosci. 26, 4289.
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- Marker, C.L. et al. (2005) J. Neurosci. 25, 3551.
- Thompson, L. et al. (2005) J. Neurosci. 25, 6467.
- Ehrlich, J.R. et al (2004) J. Physiol. 557, 583.
- Shankar, H. et al. (2004) Blood 104, 1335.
- Davila, V. et al. (2003) J. Neurosci. 23, 5693.
- Peleg, S. et al. (2002) Neuron 33, 87.
- Torrecilla, M. et al. (2002) J. Neurosci. 22, 4328.
- Inanobe, A. et al. (1999) J. Neurosci. 19, 1006.
- Pei, Q. et al. (1999) Neuroscience 90, 621.
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- GIRK2 (Kir3.2) Channel Overexpressed Membrane Fractions (#LX-102). The GIRK2 overexpressed membrane fractions enables end users to check the expression of endogenous GIRK2 as opposed to a tagged version and serves as a positive control for testing the expression of the channel and a quick way to test the antibody.
- GIRK2 (Kir3.2) Channel Antibody and Membrane Fractions Kit (#LK-102). This kit includes the GIRK2 overexpressed membrane fractions, and Anti-GIRK2 (Kir3.2) Antibody (#APC-006).
Products for control experiments
- Blockers/Antagonists: peptides/peptide toxins