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Anti-LGI1 Antibody

Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT

Cat #: ALR-041
Alternative Name Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)YKKRKINSLSPKD, corresponding to amino acid residues 206-218 of rat LGI1 (Accession Q8K4Y5). LRRCT domain.
Accession (Uniprot) Number Q8K4Y5
Gene ID 252892
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 12/13 amino acid residues identical.
RRID AB_2756663.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    1,2 Anti-LGI1 Antibody (#ALR-041), (1:200).
    3,4. Anti-LGI1 Antibody, preincubated with LGI1 Blocking Peptide (#BLP-LR041).
References
  1. Herranz-Perez, V. et al. (2010) Brain Res. 1307, 177.
  2. Leonardi, E. et al. (2011) PloS ONE 6, e18142.
  3. Irani, S.R. et al. (2010) Brain 133, 2734.
Scientific background

The leucine-rich glioma inactivated (LGI) gene subfamily contains four highly conserved members (LGI123 and 4), which have been described in human, mouse and other mammalians. LGI1 is mainly expressed in the dentate gyrus and the CA3 field of the hippocampus1.

The predicted structure of the LGI1 protein comprises, starting from the N-terminal, a signal peptide, four leucine-rich repeats (LRR) flanked on both sides by conserved cysteine clusters, and seven copies of a repeat of about 45 residues, named EPTP or EAR, probably forming a β-propeller structural domain. Both LRR and β-propeller domains mediate protein-protein interactions, each motif defining a distinct family of proteins.

LGI1 is associated with a post-synaptic complex containing PSD-95 and ADAM22, a receptor associated with the post-synaptic membrane. Through specific binding to ADAM22, LGI1 participates in the control of synaptic strength at excitatory synapses, whose malfunction may result in epilepsy.

LGI1 has been linked to several different clinical phenotypes including: malignant progression of glioma and autosomal dominant lateral temporal epilepsy, a rare familial partial epilepsy syndrome. The LGI1 gene has been shown to be frequently downregulated in malignant gliomas and to regulate invasiveness of some glioma cell lines by driving the expression of matrix metalloproteinases through the ERK 1/2 pathway. These findings suggest that LGI1 may serve as a tumor metastasis suppressor gene2. In addition, antibodies to LGI1 complexed with the KV1.1 potassium channel have been found in brain extracts from patients with limbic encephalitis3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-LGI1 Antibody (#ALR-041) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize LGI1 from human, mouse, and rat samples.

For research purposes only, not for human use
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