Anti-PSD-95 Antibody

Postsynaptic density protein 95, Synapse associated protein 90, SAP-90, Discs large homolog 4, DLG4
    Cat #: APZ-009
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95 (Accession P78352). Intracellular, between the SH3 and the GK-like domains.
    Accession (Uniprot) Number P78352
    Gene ID 1742
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1, 2. Anti-PSD-95 Antibody (#APZ-009), (1:200).
    3, 4. Anti-PSD-95 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of PSD-95 in mouse parietal cortex
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PSD-95 Antibody (#APZ-009), (1:400), followed by anti-rabbit-Cy3 antibody (red). Staining appears mainly in the pyramidal cell body (arrows). Nuclei were stained with DAPI (blue).
    References
    1. Feng, W. and Zhang, M. (2009) Nat. Rev. Neurosci. 10, 87.
    2. Scannevin, R.L. and Huganir, R.L. (2000) Nat. Rev. Neurosci. 1, 133.
    3. Doucet, M.V. et al. (2012) Pharmacol. Ther133, 218.
    4. Kalia, L.V. and Salter, M.W. (2003) Neuropharmacology 45, 720.
    5. Carlisle, H.J. et al. (2008) J. Physiol. (Lond.) 586, 5885.
    Scientific background

    The postsynaptic density (PSD) is a membrane-associated protein specialized in postsynaptic signal transduction and processing. There are four PSD family members classified according to their molecular weight, including, PSD-95, PSD-93, synaptic associated proteins 97 kDa and 102 kDa. PSD-95 interacts with both ionotropic and metabotropic glutamate receptors via protein–protein interactions and plays a role in their precise assembly and spatial organization as well as coupling of these receptors to downstream signaling events1.

    PSD-95 contains a SH3 domain, a GK domain and three PDZ domains. The PDZ domains 1 and 2 of PSD-95 are separated by only a few residues, creating a rigid bond that may restrain interdomain flexibility. These two PDZ domains can have distinct or overlapping target-binding proteins. In contrast, PDZ3 of PSD-95 is located at the carboxyl terminal end of the protein and has a set of interacting proteins that are distinct from PDZ domains 1 and 2. The N-palmitoylation of PSD-95 at Cys-3 and Cys-5 residues is essential for enhanced synaptic responses and the trafficking of PSD-95 to the plasma membrane2. With its numerous domains, PSD-95 is often referred to as a scaffold protein, which localizes and traffics various receptors, cell adhesion molecules, ion channels, kinases and phosphatases to the synaptic membrane. PSD-95 also interacts with a variety of adaptor and cytoskeleton proteins, molecular motors, as well as protein synthesis machinery3.

    PSD-95 is found at the postsynaptic membrane of excitatory synapses4.

    PSD-95 null mice have severe learning defects and exhibit both facilitation of long-term potentiation and disruption of long-term depression5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:


    Immuno-colocalization of PSD-95 and GluN2B in rat brainImmunohistochemical staining of perfusion-fixed frozen rat parietal cortex sections using Anti-PSD-95 Antibody (#APZ-009), (1:400) and Anti-NMDAR2B (GluN2B) (extracellular)-ATTO-594 Antibody (#AGC-003-AR), (1:60). A. PSD-95 staining (green). B. The same sections were stained for GluN2B (red). C. Merge of the two images shows several cells expressing both proteins (arrows point to several examples). Nuclei are stained with DAPI (blue).

    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope human PSD-95. Anti-PSD-95 Antibody (#APZ-009) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSD-95 from mouse, rat and human samples.

    For research purposes only, not for human use