- Peptide CMDLQTSLLSTGPNASN, corresponding to amino acid residues 7-20 of rat MCHR1 (Accession P97639). Extracellular, N-terminus.
- Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human SH-SY5Y (lanes 3 and 6) lysates:1-3. Anti-MCHR1 (extracellular) Antibody (#AMR-041), (1:200).
4-6. Anti-MCHR1 (extracellular) Antibody, preincubated with MCHR1 (extracellular) Blocking Peptide (#BLP-MR041).
- Expression of Melanin-concentrating hormone receptor 1 in mouse brain gliomaCell surface detection of MCHR1 in mouse brain glioma (C6) intact living cells. A. Extracellular staining of cells with Anti-MCHR1 (extracellular) Antibody (#AMR-041) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
- Kawauchi, H. et al. (1983) Nature 305, 321.
- Shimada, M. et al. (1998) Nature 396, 243.
- Chung, S. et al. (2009) Peptides 30, 1985.
- Saito, Y. et al. (1999) Nature 400, 265.
- Hill, J. et al. (2001) J. Biol. Chem. 276, 20125.
- An, S. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 7576.
- Roy, M. et al. (2006) Neuropsychopharmacology 31, 112.
- Smith, D.G. et al. (2006) Neuropsychopharmacology 31, 1135.
- Gehlert, D.R. et al. (2009) J. Pharmacol. Exp. Ther. 329, 429.
Melanin-concentrating hormone (MCH) is a 19 amino acid peptide originally isolated from the salmon pituitary gland (in fish the length of the peptide is 17 amino acids)1. In rodents and humans the peptide is synthesized in the brain and is involved in a number of physiological effects, although it is best known for its central role in the control of appetite control. For example, central administration of MCH in mice leads to obesity and conversely, knockout of the MCH gene in mice yields lean animals with an increased metabolic rate and reduced body fat2,3.
MCH exerts its effects by binding MCHR1 and MCHR2, two receptors belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members of the family, MCHR1 and MCHR2 have seven transmembrane domains, an extracellular N-terminal and intracellular C-terminal tails. MCHR1 and MCHR2 activation both lead to an increase in intracellular Ca2+ concentration3.
In rat, MCHR1 expression is quite broad, and is strongly detected in the brain. Expression of the receptor is also seen in the eye, skeletal muscle, tongue, pituitary, intestine and lymphocytes4,5. MCHR1 is also expressed in adipose tissue where it regulates leptin secretion3. In human, MCHR1 is mostly localized to the brain and pituitary. MCHR2 displays similar localization to that of MCHR1 in the brain5,6. As MCHR2 is a pseudogene in rodents, studying its function is quite challenging.
In the brain, MCHR1 is localized in many areas that are known to be involved in various psychological disorders. Indeed, MCHR1-/- animals display antianxiety- and antidepressant-like behavior7,8. Therefore, antagonizing the activity of MCHR1 could indeed serve as a means to treat depression and obesity9.
Species reactivity key:
Anti-MCHR1 (extracellular) Antibody (#AMR-041) is a highly specific antibody directed against an epitope of the rat Melanin-concentrating hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize MCHR1 from rat, mouse, and human samples.