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Anti-MCHR1 (extracellular) Antibody

Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1

Cat #: AMR-041
Alternative Name Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CMDLQTSLLSTGPNASN, corresponding to amino acid residues 7-20 of rat MCHR1 (Accession P97639). Extracellular, N-terminus.
Accession (Uniprot) Number P97639
Gene ID 83567
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 16/17 amino acid residues identical; human - 13/17 amino acid residues identical.
RRID AB_11218957.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, lci, wb
May also work in: ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human SH-SY5Y (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human SH-SY5Y (lanes 3 and 6) lysates:
    1-3. Anti-MCHR1 (extracellular) Antibody (#AMR-041), (1:200).
    4-6. Anti-MCHR1 (extracellular) Antibody, preincubated with MCHR1 (extracellular) Blocking Peptide (#BLP-MR041).
Live cell imaging / Immunocytochemistry
  • Expression of Melanin-concentrating hormone receptor 1 in mouse brain glioma
    Expression of Melanin-concentrating hormone receptor 1 in mouse brain glioma
    Cell surface detection of MCHR1 in mouse brain glioma (C6) intact living cells. A. Extracellular staining of cells with Anti-MCHR1 (extracellular) Antibody (#AMR-041) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
References
  1. Kawauchi, H. et al. (1983) Nature 305, 321.
  2. Shimada, M. et al. (1998) Nature 396, 243.
  3. Chung, S. et al. (2009) Peptides 30, 1985.
  4. Saito, Y. et al. (1999) Nature 400, 265.
  5. Hill, J. et al. (2001) J. Biol. Chem. 276, 20125.
  6. An, S. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 7576.
  7. Roy, M. et al. (2006) Neuropsychopharmacology 31, 112.
  8. Smith, D.G. et al. (2006) Neuropsychopharmacology 31, 1135.
  9. Gehlert, D.R. et al. (2009) J. Pharmacol. Exp. Ther. 329, 429.
Scientific background

Melanin-concentrating hormone (MCH) is a 19 amino acid peptide originally isolated from the salmon pituitary gland (in fish the length of the peptide is 17 amino acids)1. In rodents and humans the peptide is synthesized in the brain and is involved in a number of physiological effects, although it is best known for its central role in the control of appetite control. For example, central administration of MCH in mice leads to obesity and conversely, knockout of the MCH gene in mice yields lean animals with an increased metabolic rate and reduced body fat2,3.

MCH exerts its effects by binding MCHR1 and MCHR2, two receptors belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members of the family, MCHR1 and MCHR2 have seven transmembrane domains, an extracellular N-terminal and intracellular C-terminal tails. MCHR1 and MCHR2 activation both lead to an increase in intracellular Ca2+ concentration3.

In rat, MCHR1 expression is quite broad, and is strongly detected in the brain. Expression of the receptor is also seen in the eye, skeletal muscle, tongue, pituitary, intestine and lymphocytes4,5. MCHR1 is also expressed in adipose tissue where it regulates leptin secretion3. In human, MCHR1 is mostly localized to the brain and pituitary. MCHR2 displays similar localization to that of MCHR1 in the brain5,6. As MCHR2 is a pseudogene in rodents, studying its function is quite challenging. 

In the brain, MCHR1 is localized in many areas that are known to be involved in various psychological disorders. Indeed, MCHR1-/- animals display antianxiety- and antidepressant-like behavior7,8. Therefore, antagonizing the activity of MCHR1 could indeed serve as a means to treat depression and obesity9.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-MCHR1 (extracellular) Antibody (#AMR-041) is a highly specific antibody directed against an epitope of the rat Melanin-concentrating hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize MCHR1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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