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Anti-NPY1R (extracellular) Antibody

Neuropeptide Y receptor type 1, NPYR, NPYY1

Cat #: ANR-027
Alternative Name Neuropeptide Y receptor type 1, NPYR, NPYY1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CFDKFPSDSHRLSY, corresponding to amino acid residues 197-210 of mouse NPY1R (Accession Q04573). 2nd extracellular loop.
Accession (Uniprot) Number Q04573
Gene ID 18166
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; human – 13/14 amino acid residues identical.
RRID AB_2876807
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1, 2. Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200).
    3, 4. Anti-NPY1R (extracellular) Antibody, preincubated with NPY1R (extracellular) Blocking Peptide (#BLP-NR027).
  • Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):
    1, 2. Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200).
    3, 4. Anti-NPY1R (extracellular) Antibody, preincubated with NPY1R (extracellular) Blocking Peptide (#BLP-NR027).
Immunohistochemistry
  • Expression of NPY1R in rat striatum and subventricular zone (SVZ)
    Expression of NPY1R in rat striatum and subventricular zone (SVZ)
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NPY1R immunoreactivity (green) appears in the striatum (STR) and the SVZ. Cell nuclei were stained with DAPI (blue). LV = Lateral ventricle.
Indirect flow cytometry
  • Cell surface detection of NPY1R in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of NPY1R in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-NPY1R (extracellular) Antibody (#ANR-027), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Yang, Z. et al. (2018) Nature 556, 520.
  2. Comeras, L.B. et al. (2019) Ann. N.Y. Acad. Sci. 1455, 59.
  3. Fetissov, S.O. et al. (2004) Neuropeptides 38, 175.
  4. Dumont, Y. et al. (1998) J. Comp. Neurol. 402, 372.
  5. Holzer, P. et al. (2012) Neuropeptides 46, 261.
Scientific background

Neuropeptide Y1 receptor (NPY1R) is one out of four receptors (NPY1R, NPY2R, NPY4R and NPY5R) that bind neuropeptide Y (NPY), an abundant neuropeptide in the mammalian central and peripheral nervous systems.

NPY1R is part of the β-branch of class A G-protein coupled receptors and likewise, has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1,2.

NPY belongs to a family of gut-brain peptides that demonstrate both hormonal functions by paracrine and endocrine release in the gastrointestinal tract and a neurotransmitter functions in sympathetic ganglia and the CNS. It is highly expressed throughout the brain with particularly high expression in limbic brain areas, such as the hippocampus, amygdala, and hypothalamus3,4. NPY modulates multiple physiological and pathophysiological processes: it increases food intake and reduces fear and anxiety by activating NPY1R1,2.

Because of the multiple functions of NPY, NPY1R has great importance in the development and progression of both metabolic and neurological diseases such as diabetes, obesity, breast cancer and bone loss and indeed a number of peptides and small-molecule compounds have been characterized as NPY1R antagonists and have shown clinical potential in the treatment of these pathological states5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-NPY1R (extracellular) Antibody (#ANR-027) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NPY1R from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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