Anti-SCN4A (NaV1.4) Antibody

Sodium channel protein type 4 subunit α, SkM1, Sodium channel protein skeletal muscle subunit α, HYKPP
    Cat #: ASC-020
    Alternative Name Sodium channel protein type 4 subunit α, SkM1, Sodium channel protein skeletal muscle subunit α, HYKPP
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide EDKELKDNHILNHVG(C), corresponding to amino acid residues 877-891 of rat NaV1.4 (Accession P15390). Intracellular loop between domains II and III.
    • Anti-SCN4A (NaV1.4) Antibody
    Accession (Uniprot) Number P15390
    Gene ID 25722
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 15/17 amino acid residues identical; human - 14/17 amino acid residues identical.
    RRID AB_2040009.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, wb
    May also work in: ifc*, ih*, ip*
    Western blot
    • Anti-SCN4A (NaV1.4) Antibody
      Western blot analysis of rat skeletal muscle lysate:
      1. Anti-SCN4A (NaV1.4) Antibody (#ASC-020), (1:200).
      2. Anti-SCN4A (NaV1.4) Antibody, preincubated with the negative control antigen.
    • Human cardiac cells (Baroni, D. et al. (2013) J. Bioenerg. Biomembr. 45, 353.). 

      Mouse muscle lysate (Boyer, J.G. et al. (2013) Skelet. Muscle 3, 24.).
    Immunocytochemistry
    • Mouse LVA myocytes (1:100) (Ednie, A.R. et al. (2013) J. Mol. Cell. Cardiol. 59, 117.).
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
    3. Fjell, J. et al. (2000) NeuroReport 11, 199.
    4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
    5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    6. Isom, L.L. (2001) Neuroscientist 7, 42.
    7. Catterall, W.A. et al. (2005) Pharmacol. Rev. 57, 397.
    8. Sasaki, R. et al. (1999) Arch. Neurol. 56, 692.
    Scientific background

    Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.2-3

    Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6

    The isoform NaV1.4, is primarily expressed in skeletal muscle.7 Different missense mutations in the gene for the NaV1.4 are correlated with several muscular diseases such as Paramyotonia congenital (PMC), PMC without cold paralysis, potassium-aggravating myotonia, and hyperkalemic periodic paralysis.8

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-SCN4A (NaV1.4) Antibody (#ASC-020) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize NaV1.4 from rat, human, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Mouse muscle lysate (1:200).
      Khogali, S. et al. (2015) Physiol. Rep. 3, e12656.
    2. Mouse muscle lysate (1:200).
      Lucas, B. et al. (2014) Physiol. Genomics 46, 385.
    3. Mouse muscle lysate.
      Boyer, J.G. et al. (2013) Skelet. Muscle 3, 24.
    4. Human cardiac cells.
      Baroni, D. et al. (2013) J. Bioenerg. Biomembr. 45, 353.
    Immunocytochemistry citations
    1. CHO transfected cells.
      Ghovanloo, M.R. et al. (2018) Sci. Rep. 8, 6304.
    2. Mouse LVA myocytes (1:100).
      Ednie, A.R. et al. (2013) J. Mol. Cell. Cardiol. 59, 117.
    Shipping and Ordering information