Overview
- Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
Western blot analysis of rat DRG lysate:1. Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#ASC-016-GP), (1:200).
2. Guinea pig Anti-NaV1.8 (SCN10A) Antibody, preincubated with Nav1.8/SCN10A Blocking Peptide (#BLP-SC016).
Expression of NaV1.8 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#ASC-016-GP). A. NaV1.8 staining (green) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.
- Wu, L. et al. (2002) NeuroReport 13, 2547.
- Baker, M.D. et al. (2001) Trends Pharmacol. Sci. 22, 27.
- Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
- Fang, X. et al. (2002) J. Neurosci. 22, 7425.
- Fjell, J. et al. (2000) NeuroReport 11, 199.
- Renganathan, M. et al. (2003) Brain Res. 959, 235.
Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.92,3. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels4,5. The expression of the α subunit isoform is developmentally regulated and tissue specific.
Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons4-6. TTX-resistant channels have been suggested to play an important role in nociceptive transmission.
Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models6.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat NaV1.8 channel. Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#ASC-016-GP), raised in guinea pigs can be used in western blot and immunohistochemistry applications. It has been designed to recognize NaV1.8 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-NaV1.8 (SCN10A) Antibody (#ASC-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
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