Anti-NaV1.8 (SCN10A) Antibody

PN3, SNS, Sodium channel protein type 10 subunit alpha
    Cat #: ASC-016
    Alternative Name PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
    May also work in: m*
    Immunogen
    • Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
    • Anti-NaV1.8 (SCN10A) Antibody
    Accession (Uniprot) Number Q63554
    Gene ID 29571
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.
    RRID AB_2040188.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.9 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Anti-NaV1.8 (SCN10A) Antibody
      Western blot analysis of rat DRG lysates:
      1. Anti-NaV1.8 (SCN10A) Antibody (#ASC-016), (1:200).
      2. Anti-NaV1.8 (SCN10A) Antibody, preincubated with the negative control antigen.
    Immunoprecipitation
    • Rat dorsal root ganglia (15 µg) (Tan, Z.Y. et al. (2014) J. Neurosci. 34, 7190.).
    Immunohistochemistry
    • Anti-NaV1.8 (SCN10A) Antibody
      Expression of NaV1.8 in rat DRG
      Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-NaV1.8 (SCN10A) Antibody (#ASC-016). NaV1.8 staining (red) is cytoplasmic and the intensity varies among DRG cells. There is a partial overlap in the distribution of NaV1.8 and neurofilament 200 (green).
    • Human neuromas (1:100) (Black, J.A. et al. (2008) Ann. Neurol. 64, 644.).
    Immunocytochemistry
    • Rat dorsal root ganglia (DRG) (1:500) (Belkouch, M. et al. (2011) J. Neurosci. 31, 18381.).
    References
    1. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    2. Renganathan, M. et al. (2003) Brain Res. 959, 235.
    3. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
    4. Wu, L. et al. (2002) Neuro. Report 13, 2547.
    5. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
    6. Fjell, J. et al. (2000) Neuro. Report 11, 199.
    Scientific background

    Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 Expression of the α subunit isoform is developmentally and tissue specific.

    Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.

    Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Nav1.8 (SCN10A) Antibody
    Expression of NaV1.8 in rat DRG and sciatic nerve.Immunohistochemical staining of rat DRG and sciatic nerve using Anti-NaV1.8 (SCN10A) Antibody (#ASC-016). A. NaV1.8 (red) and KIF5B co-localize in DRG and sciatic nerve. B. NaV1.8 (red) and KIF5B expression increases following inflammation induction.Adapted from Su, Y.Y. et al. (2013) with permission of the Society for Neuroscience.
    Last update: 24/01/2020

    Anti-NaV1.8 (SCN10A) Antibody (#ASC-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize NaV1.8 from rat, human, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Rat brain neurolemma lysate.
      Murenzi, E. et al. (2017) Neurotoxicology 60, 260.
    2. Rat DRG lysate (1:200).
      Yan, J. et al. (2016) Eur. Spine J. 25, 177.
    3. Rat trigeminal ganglion lysate.
      Yang, K.Y. et al. (2016) J. Dent. Res. 95, 1183.
    4. Rat DRG lysate (1:200).
      Cheng, K.I. et al. (2014) Eur. J. Pain 18, 162.
    5. Rat DRG lysate (1:200).
      Hu, S. et al. (2013) Am. J. Physiol. 304, G311.
    6. Rat colon lysates.
      Hu, S. et al. (2013) Exp. Neurol. 248, 275.
    7. Rat sciatic nerve and rat DRG (1:2000).
      Su, Y.Y. et al. (2013) J. Neurosci. 33, 17884.
    8. Rat DRG lysates (1:200).
      Shen, K.F. et al. (2013) Exp. Neurol. 247, 466.
    9. Rat DRG lysates (1:100).
      Sun, W. et al. (2012) Brain 135, 359.
    10. Rat L4-6 DRG lysates (1:200).
      Yu, Y.Q. et al. (2011) PLoS ONE 6, e19865.
    Immunoprecipitation citations
    1. Rat dorsal root ganglia (15 µg).
      Tan, Z.Y. et al. (2014) J. Neurosci. 34, 7190.
    Immunohistochemistry citations
    1. Rat DRG sections (1:300).
      Qiu, F. et al. (2016) Neurochem. Res. 41, 1587.
    2. Mouse lumbar and DRG sections (1:150).
      Lu, R. et al. (2015) J. Neurosci. 35, 1125.
    3. Rat DRG.
      Cheng, K.I. et al. (2014) Eur. J. Pain 18, 162.
    4. Rat DRG sections (1:200).
      Hu, S. et al. (2013) Am. J. Physiol. 304, G311.
    5. Rat DRG sections(1:1000).
      Su, Y.Y. et al. (2013) J. Neurosci. 33, 17884.
    6. Rat DRG sections (1:400).
      Sun, W. et al. (2012) Brain 135, 359.
    7. Rat L4-6 DRG sections (1:200).
      Yu, Y.Q. et al. (2011) PLoS ONE 6, e19865.
    8. Rat L4-6 DRG sections (1:500).
      Belkouch, M. et al. (2011) J. Neurosci. 31, 18381.
    9. Human neuromas (1:100).
      Black, J.A. et al. (2008) Ann. Neurol. 64, 644.
    Immunocytochemistry citations
    1. ND7-23 cells (1:1000).
      Su, Y.Y. et al. (2013) J. Neurosci. 33, 17884.
    2. Rat DRGs (1:200).
      Shen, K.F. et al. (2013) Exp. Neurol. 247, 466.
    3. Rat cultured DRGs (1:500).
      Belkouch, M. et al. (2011) J. Neurosci. 31, 18381.
    More product citations
    1. Qu, R. et al. (2013) Am. J. Physiol. 304, G763.
    2. Wang, Y. et al. (2012) PLoS ONE 7, e53165.
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