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Anti-Rat SLC4A5 (NBC4) Antibody

Electrogenic sodium bicarbonate cotransporter 4, NBCe2

Cat #: ANT-076
Alternative Name Electrogenic sodium bicarbonate cotransporter 4, NBCe2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
Immunogen
  • Peptide (C)KLEPTSYRRRHPE, corresponding to amino acid residues 11-23 of rat NBCe2 (Accession Q6RI88). Intracellular, N-terminal.
Accession (Uniprot) Number Q6RI88
Gene ID 297386
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat only.
RRID AB_2341020.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for human and mouse samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 3) and testis (lanes 2 and 4) membranes:
    Western blot analysis of rat brain (lanes 1 and 3) and testis (lanes 2 and 4) membranes:
    1,2. Anti-Rat SLC4A5 (NBC4) Antibody (#ANT-076), (1:400).
    3,4. Anti-Rat SLC4A5 (NBC4) Antibody, preincubated with Rat SLC4A5/NBC4 Blocking Peptide (#BLP-NT076).
References
  1. Romero, M.F. et al. (2013) Mol. Aspects Med. 34, 159.
  2. Pushkin A. et al. (2000) Biochim. Biophys. Acta 1493, 215.
  3. Virkki, L.V. et al. (2002) Am. J. Physiol. 282, C1278.
  4. Barkley, R.A. et al. (2004) Hypertension 43, 477.
  5. Taylor, J.Y. et al. (2009) Biol. Res. Nurs. 11, 53.
  6. Kao, L. et al. (2011) J. Biol. Chem. 286, 32563.
Scientific background

The SLC4 transporter family, also known as the bicarbonate (HCO3-)-transporter family, includes the products of ten human genes (SLC4A1-5; A7-11). In mammals, the SLC4 membrane proteins are critical for several physiological processes, including the carriage of carbon dioxide (CO2) from the systemic capillaries to the pulmonary capillaries, the regulation of cell volume in multiple cell types, and the regulation of intracellular pH in nearly every cell of the body1.

NBCe2 (NBC4) is an electrogenic sodium bicarbonate co-transporter encoded by SLC4A5. There are six NBCe2 isoform (SLC4A5A-F) reported2. NBCe2 is an integral membrane protein consisting of 14 transmembrane domains. NBCe2 has a long N-terminal hydrophilic domain and a much shorter C-terminal hydrophilic domain, both of which are intracellular3.

NBCe2 is predominantly detected in the brain (highest in prefrontal cortex), epididymis, cardiac muscle, smooth muscle, kidney, and choroid plexus/apical1.

Several studies identified SLC4A5 as a positional candidate for hypertension4,5. In mice, disruption of slc4a5 causes both arterial hypertension as well as metabolic acidosis (distal renal tubular acidosis)6. In addition, mice lacking NBCe2 have reduced choroid plexus secretion and reduced ventricle size. These combined issues change intracranial pressure, CSF electrolytes, and cause severe eye pathologies such as photoreceptor loss, ganglion cell loss, and retinal detachment7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NBCe2. Anti-Rat SLC4A5 (NBC4) Antibody (#ANT-076) can be used in western blot analysis. It has been designed to recognize NBCe2 from rat samples. The antibody is not recommended for mouse and human samples.

For research purposes only, not for human use
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