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- Peptide (C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2 (Accession P48763). Intracellular, C-terminus.
- Western blot analysis of rat kidney membranes (lanes 1 and 4), mouse kidney lysates (lanes 2 and 5) and rat stomach lysates (lanes 3 and 6):1-3. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:200).
4-6. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody, preincubated with the negative control antigen.
- Western blot analysis of human Colo-205 colorectal carcinoma cell lysates:1. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:200).
2. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody, preincubated with the negative control antigen.
- Expression of Na+/H+ exchanger 2 in rat colonImmunohistochemical staining of rat colon sections (paraffin-embedded) using Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:50). A. NHE-2 staining is shown in red. B. Cell nuclei were labeled with DAPI (blue). C. Merge of the two images.
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In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1.
To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4.
Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1,5,8.
NHE-2 is detected in the intestine, kidney and parietal cells2,9-11. It is also detected in skeletal muscle and testis2,12.
Species reactivity key:
Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NHE-2 from human, rat, and mouse samples.
- Human Barrett's esophageal cell lysate (1:200).
Laczko, D. et al. (2016) Am. J. Physiol. 311, G16.