Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody

Sodium/hydrogen exchanger 2, SLC9A2
    Cat #: ANX-002
    Alternative Name Sodium/hydrogen exchanger 2, SLC9A2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2 (Accession P48763). Intracellular, C-terminus.
        Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody
    Accession (Uniprot) Number P48763
    Gene ID 24783
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 12/16 amino acid residues identical.
    RRID AB_11219158.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody
        Western blot analysis of rat kidney membranes (lanes 1 and 4), mouse kidney lysates (lanes 2 and 5) and rat stomach lysates (lanes 3 and 6):
        1-3. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:200).
        4-6. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody, preincubated with the negative control antigen.
        Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody
        Western blot analysis of human Colo-205 colorectal carcinoma cell lysates:
        1. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:200).
        2. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody, preincubated with the negative control antigen.
      • Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody
        Expression of Na+/H+ exchanger 2 in rat colon
        Immunohistochemical staining of rat colon sections (paraffin-embedded) using Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:50). A. NHE-2 staining is shown in red. B. Cell nuclei were labeled with DAPI (blue). C. Merge of the two images.
    References
      • In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1.

        To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4.

        Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6.  K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1,5,8.

        NHE-2 is detected in the intestine, kidney and parietal cells2,9-11. It is also detected in skeletal muscle and testis2,12.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NHE-2 from human, rat, and mouse samples.

    For research purposes only, not for human use