- Peptide CRKMYNRPHRAPITK, corresponding to amino acid residues 181 - 195 of rat Netrin-1 (Accession Q924Z9). Secreted.
- Mouse and rat brain lysates; human U-87 MG glioblastoma and PANC-1 pancreas ductal adenocarcinoma cell lysates (1:200-1:1000).
- Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200).
3, 4. Anti-Netrin-1 Antibody, preincubated with Netrin-1 Blocking Peptide (#BLP-NR121).
Netrins are a family comprised of five members: three secreted proteins (netrins 1, 3 and 4), and two glycosylphosphatidylinositol (GPI)-anchored membrane proteins, netrins G1 and G21. Netrin-1 is a diffusible long-range axonal guidance cue expressed in floor plate cells of the central nervous system. It is secreted in order to direct exons to their target1-3. Axon guidance by netrin-1 is implicated in multiple developing brain regions and developing neuronal types. It also plays a role in axon branching, synaptogenesis, cell migration, cell survival, and axon regeneration4.