Anti-Semaphorin-3A Antibody

SEMA3A
Cat #: ASR-051
Alternative Name SEMA3A
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KRDRKQRRQRPGH, corresponding to amino acid residues 728-740 of rat Semaphorin-3A (Accession Q63548). C-terminus.
    Accession (Uniprot) Number Q63548
    Gene ID 29751
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ih, ip
      • Western blot analysis of mouse (lanes 1 and 3) and rat brain mebranes  (lanes 2 and 4):
        1, 2. Anti-Semaphorin-3A Antibody (#ASR-051), (1:400).
        3, 4. Anti-Semaphorin-3A Antibody, preincubated with the control peptide antigen.
        Western blot analysis of human THP-1 acute monocytic leukemia cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate  (lanes 2 and 4):
        1, 2. Anti-Semaphorin-3A Antibody (#ASR-051), (1:400).
        3, 4. Anti-Semaphorin-3A Antibody, preincubated with the control peptide antigen.
      • Semaphorins are members of a large family of proteins controlling a variety of processes in the central nervous system (CNS) during development such as cell migration and axonal growth cone guidance but are also expressed in other tissues. Their common feature is a conserved 500 amino acid “sema” domain. There are currently eight defined classes of semaphorins; classes 2 and 3 are secreted proteins while others are GPI-linked or transmembrane proteins.

        Semaphorin 3A protein is an elongated disc shaped molecule with dimensions of 60X70X45 angstrom and a molecular weight of 65 kDA. The semaphorin fold is a variation of the β propeller topology with seven blades radially arranged around a central axis. Each blade averages 70 amino acids and contains a four stranded antiparallel sheet. The large size of this protein derives from the secondary structural elements attached to each blade.

        Two units of Semaphorin 3A create a functional Semaphorin 3A dimer. Neuropilin-1 binds to semaphorin-3A and competes with semaphorin dimerization indicating that the neuropilin-1 binding region of Semaphorin 3A is in proximity to the dimerization site1. Another Semaphorin binding molecule is plexin-1. It does not bind to semaphorin alone but only in a complex with Neuropilin-1. Interestingly, this complex has higher affinity to semaphorin than Neuropilin-1 alone. In addition, binding of neuropilin-1 does not change non-neuronal cell morphology while the combination of Neuropilin-1 and plexin-1 induces an alteration in adherent cells2.

        The downregulation of Semaphorin expression was found to be linked to many pathological conditions of the CNS such as ischemia, degenerative diseases and multiple sclerosis and it was also found that semaphorin-3A encourages tumor growth in multiple myeloma3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 13/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Semaphorin-3A. Anti-Semaphorin-3A Antibody (#ASR-051) can be used in western blot analysis. It has been designed to recognize SEMA3A from mouse, rat and human samples.

    For research purposes only, not for human use