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Anti-Orexin Receptor 2 Antibody

OX2R, Hypocretin receptor type 2, HCRTR2
Cat #: AOR-002
Alternative Name OX2R, Hypocretin receptor type 2, HCRTR2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R (Accession P56719). Intracellular, C-terminus.
Accession (Uniprot) Number P56719
Gene ID 25605
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 14/15 amino acid residues identical.
RRID AB_2040050.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates:
    Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates:
    1,2.  Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:200).
    3,4.  Anti-Orexin Receptor 2 Antibody, preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:400).
    3,4. Anti-Orexin Receptor 2 Antibody, preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).
Immunohistochemistry
  • Expression of OX2R in rat brain
    Expression of OX2R in rat brain
    Immunohistochemical staining of rat brain using Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:50), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (1:500), (red). A. In the cerebellum, staining is present in cell bodies of both Golgi type I (Purkinje cells – white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML). B. In the parietal cortex, staining is evident in neural cell bodies. C. In the hippocampus, staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained. Hoechst 33342 is used as the counterstain (blue).
Immunocytochemistry
  • Expression of OX2R in human colon cancer cells
    Expression of OX2R in human colon cancer cells
    Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human Colo-205 colon cancer cells using Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:500), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Live view of the same field as in (A). C. Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue).
References
  1. Sakurai, T. et al. (1998) Cell. 92, 573.
  2. Kukkonen, J.P. et al. (2002) Am. J. Physiol. Cell. Physiol. 283, C1567.
  3. Kirchgessner, A.L. (2002) Endocr. Rev. 23, 1.
  4. Sakurai, T. (2007) Nat. Rev. Neurosci. 8, 171.
Scientific background

Orexin Receptor 2 (OX2R) (also known as hypocretin receptor 2) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.

OX2R binds both orexin A and B with similar affinities while OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference).2,3

Both OX2R and OX1R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. OX2R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels. OX2R can also transmit signals through inhibitory Gi G proteins, although the mechanism is less well understood.2,3

The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research over the last few years. OX2R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2

The best studied physiological role of OX2R is its involvement in the regulation of sleep and wakefulness states. Studies in mice lacking the orexin gene and dogs expressing a null mutation of the OX2R show a remarkably similar phenotype to human narcolepsy, a condition characterized by excessive daytime sleepiness, inability to maintain vigilant states, and defects in the regulation of rapid eye movement (REM) sleep.4

In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-Orexin Receptor 2 Antibody (#AOR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX2R from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat brain lysate.
    Zhou, J.J. et al. (2015) Neuropharmacology 99, 481.
Immunohistochemistry citations
  1. Rat brain sections.
    Yokota, S. et al. (2016) Brain Res. 1648, 512.
Immunocytochemistry citations
  1. Rat isolated rod bipolar cells (RBCs).
    Zhang, G. et al. (2018) Neuropharmacology 133, 38.
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