Orexin Receptor 2 Blocking Peptide (#BLP-OR002) is the original antigen used for immunization during Anti-Orexin Receptor 2 Antibody (#AOR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Orexin Receptor 2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates:
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Expression of OX2R in rat brainImmunohistochemical staining of rat brain using Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:50), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (1:500), (red). A. In the cerebellum, staining is present in cell bodies of both Golgi type I (Purkinje cells – white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML). B. In the parietal cortex, staining is evident in neural cell bodies. C. In the hippocampus, staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained. Hoechst 33342 is used as the counterstain (blue).
- (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R (Accession P56719).