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Anti-SLC22A1 Antibody

OCT1, Organic cation transporter 1

Cat #: ACT-011
Alternative Name OCT1, Organic cation transporter 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)ENLGRRKSKAKENT, corresponding to amino acid residues 530- 543 of rat OCT1 (Accession Q63089). Intracellular, C-terminus.
Accession (Uniprot) Number Q63089
Gene ID 24904
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical.
RRID AB_2340930.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat small intestine lysate (lanes 1 and 3) and rat H4-II-E-C3 hepatoma cell lysate (lanes 2 and 4):
    Western blot analysis of rat small intestine lysate (lanes 1 and 3) and rat H4-II-E-C3 hepatoma cell lysate (lanes 2 and 4):
    1-2. Anti-SLC22A1 Antibody (#ACT-011), (1:200).
    3-4. Anti-SLC22A1 Antibody, preincubated with SLC22A1 Blocking Peptide (#BLP-CT011).
References
  1. Rulcova, A. et al. (2013) Pharmacol. Rep. 65, 1322.
  2. Lozano, E. et al. (2013) BioMed Res. Int. 2013, 1.
  3. Gorboulev, V. et al. (1997) DNA Cell Biol. 16, 871.
  4. Meyer-Wentrup, F. (1998) Biochem. Biophys. Res. Commun. 248, 673.
  5. Lautem, A. et al. (2013) Int. J. Oncol. 42, 1297.
  6. Koepsell, H. et al. (2007) Pharm. Res. 24, 1227.
  7. Martinez-Becerra, P. et al. (2012) Mol. Pharmacol. 9, 1693.
Scientific background

Organic cation transporters belong to the Solute carrier family 22 (SLC22) and the MATE family. The SLC22 family contains the three subtypes of passive diffusion organic cation transporters called OCT1 (SLC22A1), OCT2 (SLC22A2) and OCT3 (SLC22A3). OCT1 is responsible for the uptake of organic cationic compounds from blood to hepatocytes1.
 
Like most members of the SLC22 family, the organic cation and carnitine transporters have a predicted membrane topology that comprises 12 α-helical transmembrane domains (TMDs), an intracellular N-terminus, a large glycosylated extracellular loop, a large intracellular loop with phosphorylation sites and an intracellular C-terminus2.
 
In humans, OCT1 has a broad tissue distribution, although it is primarily expressed in hepatocytes3. In rat hepatocytes, OCT1 has been located at the sinusoidal membrane4. OCT1 is also expressed in cholangiocytes5. Human OCT1 is also expressed at the apical membrane of epithelial cells in the proximal and distal tubules of the nephron6.
 
A recent study demonstrated that primary liver cancer derived from epithelial cells, namely, hepatocellular carcinoma (HCC), cholangiocarcinoma (CGC), and hepatoblastoma, shares a decreased expression of OCT1 as a common feature7. The reduction in the expression levels of this transporter has been associated with advanced tumor stages and poorer overall patient survival rates in HCC and CGC5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Organic Cation Transporter 1. Anti-SLC22A1 Antibody (#ACT-011) can be used in western blot analysis. It has been designed to recognize OCT1 from rat and mouse samples. The antibody is not recommended for human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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