Anti-SLC22A3 (extracellular) Antibody

OCT3, Organic cation transporter 3
  • New
Cat #: ACT-013
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, r
    Immunogen
    Peptide (C)ERRGQGH(S)HRYLLE, corresponding to amino acid residues 83 - 96 of rat OCT3 (Accession O88446). Extracellular, 1st loop.
    Accession (Uniprot) Number O88446
    Gene ID 29504
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse – 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Not recommended for human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
    Western blot
    Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1-2. Anti-SLC22A3 (extracellular) Antibody (#ACT-013), (1:200).
    3-4. Anti-SLC22A3 (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of OCT3 in mouse cortex and hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC22A3 (extracellular) Antibody (#ACT-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC22A3 staining (green) in mouse cortex is detected in in neurons (arrows). B. In mouse hippocampus, OCT3 immunoreactivity (green) is observed in astrocytes (vertical arrows) and neurons (horizontal arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Vollmar, J. et al. (2017) Oncotarget 8, 115667.
    2. Gasser, P.J. et al. (2017) Brain Struct. Funct. 222, 1913.
    3. Li, D.C. et al. (2015) J. Biol. Chem. 290, 27633.
    Scientific background

    Organic cation transporter 3 (SLC22A3) belongs to the SLC22A gene family. Organic cation transporters (OCTs) are membrane transport proteins involved in many metabolic processes and detoxification. The transporters are responsible for the uptake and intracellular inactivation of numerous endogenous and exogenous substrates including norepinephrine, epinephrine, dopamine, histamine and serotonin. They are also responsible for the uptake of a variety of anticancer drugs1,2. OCT structure is suggested to have 12 transmembrane domains and a large hydrophobic cleft capable of accommodating a wide variety of chemical species3.

    In humans, OCT3 is expressed in many tissues; abundantly in the placenta, heart, liver and skeletal muscle. It is also expressed in astrocytes, intestinal epithelium neurons, glia, ependymal cells, and blood-cerebrospinal fluid barrier in choroid plexus epithelial cells. The protein is also widely distributed in the brain. OCT expression is detected in several cancer cell lines and tumor tissue samples1-3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 23/10/2018

    Anti-SLC22A3 (extracellular) Antibody (#ACT-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Organic Cation Transporter 3 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

    For research purposes only, not for human use