Name: Anti-P2Y12 Receptor (extracellular)-APC Antibody
Brand: Alomone Labs
SKU: APR-020-APC

Overview

Cat #: APR-020-APC

Alternative Name P2Y purinoceptor 12, P2RY12, P2Y receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2Y12 receptor (Accession Q9H244). 3^rdextracellular loop.

Accession (Uniprot) Number Q9H244

Gene ID 64805

Homology Rat, mouse – 12/13 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Allophycocyanin (APC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-020), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of P2Y12 by direct flow cytometry in live intact mouse BV-2 microglia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-APC (#RIC-001-APC).
^___ Cells + Anti-P2Y12 Receptor (extracellular)-APC Antibody (#APR-020-APC), (2.5µg).
Cell surface detection of P2Y12 by direct flow cytometry in live intact human MEG-01 megakaryocytic cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-APC (#RIC-001-APC).
^___ Cells + Anti-P2Y12 Receptor (extracellular)-APC Antibody (#APR-020-APC), (2.5µg).
Cell surface multicolor flow cytometry analysis of live intact mouse BV-2 microglia cells with P2Y12 and TREM2:
BV-2 cells were labeled with Anti-P2Y12 Receptor (extracellular)-APC Antibody (#APR-020-APC), (5µg) and with Anti-TREM2 (extracellular)-PE Antibody (#ANR-018-PE), (5µg).

References

Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.

Scientific background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14^1-3.

The P2Y12 receptor is co-expressed with the P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca²⁺ upon activation.

The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting⁴. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize P2Y12 from human, mouse, and rat samples.

Anti-P2Y12 Receptor (extracellular)-APC Antibody (#APR-020-APC) is directly conjugated to the Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 16/04/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-APC (#RIC-001-APC), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Allophycocyanin (APC).

Rabbit IgG Isotype Control-APC (#RIC-001-APC)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-P2Y12 Receptor (extracellular)-APC Antibody