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Anti-P2Y6 Receptor (extracellular)-PE Antibody

P2Y purinoceptor 6, P2RY6

Cat #: APR-106-PE
Alternative Name P2Y purinoceptor 6, P2RY6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172 - 186 of mouse P2RY6 (Accession Q9ERK9). Extracellular, 2nd loop.
Accession (Uniprot) Number Q9ERK9
Gene ID P2ry6
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label R-Phycoerythrin (R-PE).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-106), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of P2Y6 by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of P2Y6 by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cell alone
    ___ Cells + Rabbit IgG isotype control-PE
    ___ Cells + Anti-P2Y6 Receptor (extracellular)-PE Antibody (APR-106-PE), 2.5µg.
  • Cell surface detection of P2Y6 by direct flow cytometry in live intact mouse BV-2 microglia cells:
    Cell surface detection of P2Y6 by direct flow cytometry in live intact mouse BV-2 microglia cells:
    ___ Cell alone
    ___ Cells + Rabbit IgG isotype control-PE
    ___ Cells + Anti-P2Y6 Receptor (extracellular)-PE Antibody (APR-106-PE), 2.5µg.
References
  1. Communi, D. et al. (2000) Cell Signal. 12, 351.
  2. Filippov, A.K. et al. (1999) Br. J. Pharmacol. 126, 1009
  3. Chang, K. et al. (1995) J. Biol. Chem. 270, 26152.
  4. Malmsjo, M. et al. (2003) B.M.C. Pharmacol. 3, 4.
Scientific background

The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily1,3.

P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels1,2.

P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize P2RY6 from human, rat, and mouse samples.

Anti-P2Y6 Receptor (extracellular)-PE Antibody (#APR-106-PE) is directly conjugated to the Phycoerythrin (PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

For research purposes only, not for human use
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