Name: Anti-P2Y2 Receptor (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: APR-102-F

Overview

Cat #: APR-102-F

Alternative Name P2Y purinoceptor 2, P2RY2, P2U1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (CD)TINGTWEGDELGYK, corresponding to amino acid residues 11-24 of rat P2RY2 (Accession P41232). Extracellular, C-terminus.

Accession (Uniprot) Number P41232

Gene ID 29597

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.

RRID AB_2756770.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-102), and direct flow cytometry (labeled antibody).

Applications: fc, lci

May also work in: ic*

Direct flow cytometry

Cell surface detection of P2RY2 in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-P2Y2 Receptor (extracellular)-FITC Antibody (#APR-102-F), (2.5 µg).
Cell surface detection of P2RY2 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-P2Y2 Receptor (extracellular)-FITC Antibody (#APR-102-F), (5 µg).

References

Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
Di Virgilio, F. et al. (2001) Blood 97, 587.
Yerxa, B.R. et al. (2002) J. Pharmacol. Exp. Ther. 302, 871.

Scientific background

P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation¹.

The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14^2-4.

The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway⁵. It has become a leading target for the treatment of cystic fibrosis. Activation of P2RY2 increases Cl^- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize P2RY2 from rat, mouse, and human samples.

Anti-P2Y2 Receptor (extracellular)–FITC Antibody (#APR-102-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 10/04/2023

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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Anti-P2Y2 Receptor (extracellular)-FITC Antibody