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Anti-PMCA4 Antibody

Plasma membrane Ca2+ ATPase 4, Matrix-Remodeling-Associated Protein 1, ATP2B4

Cat #: ACP-004
Alternative Name Plasma membrane Ca2+ ATPase 4, Matrix-Remodeling-Associated Protein 1, ATP2B4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
May also work in: m*
Immunogen
  • Peptide CDRILNKEGGIVPFKTK, corresponding to amino acid residues 601-617 of rat PMCA4 (Accession Q64542). 2nd intracellular loop.
Accession (Uniprot) Number Q64542
Gene ID 29600
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 12/17 amino acid residues identical.
RRID AB_2756566.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity This antibody is not recommended for human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat stomach lysate (lanes 1 and 3) and rat lung membranes (lanes 2 and 4):
    Western blot analysis of rat stomach lysate (lanes 1 and 3) and rat lung membranes (lanes 2 and 4):
    1,2. Anti-PMCA4 Antibody (#ACP-004), (1:200).
    3,4. Anti-PMCA4 Antibody, preincubated with PMCA4 Blocking Peptide (#BLP-CP004).
Immunohistochemistry
  • Expression of PMCA4 in rat parietal cortex
    Expression of PMCA4 in rat parietal cortex
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA4 Antibody (#ACP-004), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. PMCA4 staining (green) appears in neuronal profiles in deep layers of the parietal cortex (arrows). Nuclei are labeled with DAPI (blue).
References
  1. Strehler, E.E. and Zacharias, D.A. (2001) Physiol. Rev. 81, 21.
  2. Schuh, K. et al. (2004) J. Biol. Chem. 279, 28220.
Scientific background

Plasma membrane calcium pumps (PMCA) are part of the P2 subfamily of P-type primary ion transport ATPases. PMCAs are found in all eukaryotic cells and serve as a major high-affinity transporter for Ca2+ in the plasma membrane.

PMCA4, as other PMCA pumps, is predicted to have 10 membrane spanning domains with the -NH2 and -COOH termini both located on the cytosolic side of the membrane. Most of the protein is facing the cytosol and is comprised of three main parts - the intracellular loop between transmembrane segments 2 and 3, the large unit between membrane-spanning domains 4 and 5, and an extended “tail” following the last transmembrane domain.

PMCA4 is regulated by various regulatory mechanisms including Ca2+-Calmodulin and acidic phospholipids. Calmodulin binds to a region in the -COOH terminal located about 40 residues downstream from the last transmembrane domain. In the absence of calmodulin, this region acts as an auto-inhibitory domain. Acidic phospholipids are strong activators of PMCA4. They reduce the Michaelis-Menten constant for Ca2+ of the enzyme to roughly 0.3 µM compared with 0.4–0.7 µM in the case of calmodulin activation. Activation by phospholipids makes the PMCA insensitive to calmodulin thus suggesting that their effect is not additive1.

PMCA4 deficiency does not affect embryonic development in mice. PMCA4 knockout mice are indistinguishable from wild type mice in terms of their behavior, appearance and weight. PMCA4 is highly expressed in the sperm tail and deletion of PMCA4 causes severely impaired sperm motility in mice2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat PMCA4. Anti-PMCA4 Antibody (#ACP-004) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA4 from rat and mouse samples. It is unlikely to recognize PMCA4 from human samples.

For research purposes only, not for human use
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