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Anti-Secretin Receptor (extracellular) Antibody

SCTR
Cat #: AGR-026
Alternative Name SCTR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)NSFNERRHAYLLKLK, corresponding to amino acid residues 129 - 143 of rat Secretin Receptor (Accession P23811). Extracellular, N-terminus.
Accession (Uniprot) Number P23811
Gene ID 6344
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat pancreas lysate (lanes 3 and 7) and mouse kidney membranes (lanes 4 and 8):
    Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat pancreas lysate (lanes 3 and 7) and mouse kidney membranes (lanes 4 and 8):
    1-4. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200).
    5-8. Anti-Secretin Receptor (extracellular) Antibody, preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).
  • Western blot analysis of human PANC-1 pancreatic carcinoma cell lysate:
    Western blot analysis of human PANC-1 pancreatic carcinoma cell lysate:
    1. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200).
    2. Anti-Secretin Receptor (extracellular) Antibody, preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).
Immunohistochemistry
  • Expression of Secretin Receptor in mouse cerebellum
    Expression of Secretin Receptor in mouse cerebellum
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Secretin Receptor immunoreactivity (green) appears in Bergmann glia profiles (vertical arrows point at Bergmann cell bodies and horizontal arrows at Bergmann glia processes). B. Pre-incubation of the antibody with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026), suppresses staining. Cell nuclei are stained with DAPI (blue).
  • Expression of Secretin Receptor in rat hippocampus
    Expression of Secretin Receptor in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Secretin Receptor immunoreactivity (green) appears in astrocyte profiles (arrows). G = granule layer. OML = outer molecular layer. B. Pre-incubation of the antibody with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026), suppresses staining. Cell nuclei are stained with DAPI (blue).
References
  1. Bai, J.J. et al. (2017) Am. J. Physiol. 312, F852.
  2. Holtmann, M.H. et al. (1995) J. Biol. Chem. 270, 14394.
  3. Afroze, S. et al. (2013) Ann. Transl. Med. 3, 29.
Scientific background

Secretin receptor, SCTR, binds Secretin, a hormone that affects water homeostasis throughout the body and specifically in the duodenum of the small intestine.

SCTR is ubiquitously expressed throughout the body. It belongs to class B1 of G-protein coupled receptors, along with vasoactive intestinal polypeptide (VIP) receptor. Like all G-protein coupled receptors, it is composed of seven highly conserved transmembrane domains1.  The amino terminus of Secretin receptor plays a key role in agonist recognition and responsiveness2.

The binding of SCTR to Secretin initiates a signaling cascade that stimulates bicarbonate secretion which in turn leads to neutralization of gastric H+. This process plays an essential role in fat digestion by creating a more neutral (pH 6 to 8) environment.  Secretin levels are regulated by H+ and fatty acids levels in the duodenum and hence the secretion regulation is tightly bound to its activity3.

In addition, SCTR-Secretin interaction plays a major role in the regulation of the secretory activity in the heart, liver and the central nervous system (CNS)3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 07/09/2020

Anti-Secretin Receptor (extracellular) Antibody (#AGR-026) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SCTR from human, mouse, and rat samples.

For research purposes only, not for human use
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