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Anti-Glucagon Receptor (extracellular) Antibody

GCGR, GLR

Cat #: AGR-024
Alternative Name GCGR, GLR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KTRYSQKIGDDLS, corresponding to amino acid residues 200-212 of rat GCGR (Accession P30082). 1st  extracellular loop.
Accession (Uniprot) Number P30082
Gene ID 24953
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 12/13 amino acid residues identical.
RRID AB_2340975.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 5), rat liver (lanes 2 and 6), mouse liver (lanes 3 and 7) and human HepG2 liver carcinoma cell line (lanes 4 and 8) lysates: 
    Western blot analysis of rat kidney (lanes 1 and 5), rat liver (lanes 2 and 6), mouse liver (lanes 3 and 7) and human HepG2 liver carcinoma cell line (lanes 4 and 8) lysates
    1-4. Anti-Glucagon Receptor (extracellular) Antibody (#AGR-024), (1:200).
    5-8. Anti-Glucagon Receptor (extracellular) Antibody, preincubated with Glucagon Receptor (extracellular) Blocking Peptide (#BLP-GR024).
Immunohistochemistry
  • Expression of Glucagon receptor in mouse hypothalamus
    Expression of Glucagon receptor in mouse hypothalamus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Glucagon Receptor (extracellular) Antibody (#AGR-024), (1:200), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. GCGR staining (green) in mouse hypothalamus is detected in neurons (arrows) from the 3rd ventricle wall outlining the paraventricular nucleus (PVN). Cell nuclei are stained with DAPI (blue).
References
  1. Authier, F. and Desbuquois, B. (2008) Cell. Mol. Life Sci. 65, 1880.
  2. Siu, F.Y. et al. (2013) Nature 499, 444.
  3. Brubaker, P.L. et al. (2002) Receptors and Channels 8, 179.
  4. Zhou, C. et al. (2009) Pancreas 38, 941.
  5. Cho, Y.M. et al. (2012) Pharmacol. Ther. 135, 247.
Scientific background

The glucagon receptor (GCGR) is one of the 15 members of the secretin-like (class B) family of G-protein-coupled receptors (GPCRs) in humans. Glucagon is a highly conserved 29 amino acid protein processed from proglucagon by PC2 in pancreatic α-cells. The principal actions of glucagon involve regulation of metabolic pathways involved in glucose homeostasis1. Glucagon binding to the GCGR activates adenylyl cyclase through heterotrimeric Gs G proteins, leading to an increase in intracellular cAMP levels and subsequent activation of PKA signaling2.
The glucagon receptor is a 485-amino acid protein with a central core consisting of seven membrane-spanning domains, an extracellular N-terminus and a cytoplasmic C-terminal tail2.

Glucagon receptors are widely expressed in multiple tissues including the liver, brain, pancreas, heart, kidney, and smooth muscle cell in the gastrointestinal tract and peripheral vasculature1.

Abnormal glucagon signaling is associated with hyperglycemia in patients with type 2 diabetes3. Mice with homozygous null mutation of GCGR exhibit chronic hypoglycemia, extreme hyperglucagonemia, and α cell hyperplasia4. GCGR is a potential drug target for type 2 diabetes5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glucagon receptor. Anti-Glucagon Receptor (extracellular) Antibody (#AGR-024) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GCGR from rat, mouse and human samples.

For research purposes only, not for human use
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