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Anti-SLC17A5 (Sialin) Antibody

Sialic acid transporter, H+/sialic acid cotransporter, Vesicular H+/aspartate-glutamate cotransporter

Cat #: AST-015
Alternative Name Sialic acid transporter, H+/sialic acid cotransporter, Vesicular H+/aspartate-glutamate cotransporter
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KGEVQSWALSDHHG, corresponding to amino acid residues 479-492 of mouse SLC17A5 (Accession Q8BN82). Intracellular, C-terminus.
Accession (Uniprot) Number Q8BN82
Gene ID 235504
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 13/14 amino acid residues identical; human – 12/14 amino acid residues identical.
RRID AB_2756802.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat small intestine (lanes 1 and 3) and brain (lanes 2 and 4) lysates:
    Western blot analysis of rat small intestine (lanes 1 and 3) and brain (lanes 2 and 4) lysates:
    1,2. Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200).
    3,4. Anti-SLC17A5 (Sialin) Antibody, preincubated with SLC17A5/Sialin Blocking Peptide (#BLP-ST015).
  • Western blot analysis of mouse colon lysate:
    Western blot analysis of mouse colon lysate:
    1. Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200).
    2. Anti-SLC17A5 (Sialin) Antibody, preincubated with SLC17A5/Sialin Blocking Peptide (#BLP-ST015).
  • Western blot analysis of human HT-29 colorectal adenocarcinoma (lanes 1 and 3) and human HepG2 liver hepatome (lanes 2 and 4) cell lysates:
    Western blot analysis of human HT-29 colorectal adenocarcinoma (lanes 1 and 3) and human HepG2 liver hepatome (lanes 2 and 4) cell lysates:
    1,2. Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200).
    3,4. Anti-SLC17A5 (Sialin) Antibody, preincubated with SLC17A5/Sialin Blocking Peptide (#BLP-ST015).
Immunohistochemistry
  • Expression of Sialin in rat spinal cord.
    Expression of Sialin in rat spinal cord.
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. SLC17A5 immunoreactivity (green) appears in the neuropil of the ventral horn. Cell nuclei are stained with DAPI (blue).
References
  1. Tarailo-Graovac, M. et al. (2017) Orphanet. J. Rare Dis. 12, 28.
  2. Lines, M.A. et al. (2014) JIMD Rep. 12, 79.
  3. Courville, P. et al. (2010) J. Biol. Chem. 285, 19316.
Scientific background

Sialin, encoded by the SLC17A5 gene, is a member of the SLC17 solute carrier family, a group of structurally related polytopic membrane proteins, part of the major facilitator superfamily of transporters. Sialin is a sialic acid transporter located in the lysosomal membrane. In the lysosome, sialic acid residues are sequentially removed from the carbohydrate chain by sialidases and then transported out of the lysosome by the transporter protein sialin1,2. Sialin structure consists of 12 transmembrane helices (TMs). TM4 contains three residues (Arg-168, Glu-175, and His-183) that are highly conserved in the SLC17 family, suggesting that they may participate directly in substrate binding and translocation. In addition, TM4 contains a GXXXG helix packing motif that is important for the expression of sialin. SLC17 family includes several more GXXXG-like motifs located within TMs including GXXXG/S in TM2, G/AXXXG in TM5, and G/S/AXXXGXXXG/T in TM13.

Proteins included in the SLC17 family are implicated with inherited neurological and metabolic diseases. Mutations in the SLC17A5 gene cause sialic acid storage diseases including infantile sialic acid storage disease and Salla disease. These conditions are autosomal recessive neurodegenerative disorders characterized by excessive accumulation of sialic acid in the lysosome1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Anti-SLC17A5 (Sialin) Antibody (#AST-015) is a highly specific antibody directed against an epitope of the mouse Sialin transporter. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Sialin from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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