Free shipping starts now, no minimum, no coupons required!

Anti-SLC41A2 (extracellular) Antibody

Solute carrier family 41 member 2, Solute carrier family 41 (magnesium transporter), Member 2

Cat #: ANT-146
Alternative Name Solute carrier family 41 member 2, Solute carrier family 41 (magnesium transporter), Member 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KRSEPHLDYHEFSE, corresponding to amino acid residues 78-91 of mouse SLC41A2 (Accession Q8BYR8). Extracellular, N-terminus.
Accession (Uniprot) Number Q8BYR8
Gene ID 338365
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat- identical; human – 9/14 amino acid residues identical.
RRID AB_2876810
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:
    1, 2. Anti-SLC41A2 (extracellular) Antibody (#ANT-146), (1:2000).
    3, 4. Anti-SLC41A2 (extracellular) Antibody, preincubated with SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146).
  • Western blot analysis of human liver carcinoma HepG2 cell line lysate (lanes 1 and 2):
    Western blot analysis of human liver carcinoma HepG2 cell line lysate (lanes 1 and 2):
    1. Anti-SLC41A2 (extracellular) Antibody (#ANT-146), (1:1000).
    2. Anti-SLC41A2 (extracellular) Antibody, preincubated with SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146).
Immunohistochemistry
  • Expression of SLC41A2 in mouse midbrain.
    Expression of SLC41A2 in mouse midbrain.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC41A2 (extracellular) Antibody (#ANT-146), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the substantia nigra region, showed SLC41A2 immunoreactivity (green) in the substantia nigra pars compacta (SNC). B. Pre-incubation of the antibody with SLC41A2 (extracellular) Blocking Peptide (BLP-NT146), suppressed staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.
References
  1. Schweigel-Röntgen, M. and Kolisek, M. (2014) Curr. Top. Membr. 73, 383.
  2. Sahni, J. and Scharenberg, A.M. (2013) Mol. Aspects Med. 34, 620.
  3. Wabakken, T. et al. (2003) Biochem. Biophys. Res. Commun. 306, 718.
Scientific background

SLC41A2, Solute carrier family 41 member 2, is a magnesium (Mg2 +) transporter belonging to the SLC41 transporter family, which includes SLC41A1 and SLC41A31.

SLC41 proteins appear to possess the same basic structural features of prokaryotic MgtE transporters: they create a Mg2+-selective pore via homodimerization. SLC41 topology suggests the presence of two five TM span domains connected by a TM spanning linker. The N-terminal cytoplasmic domain of SLC41A1 regulates its function2,3.

SLC41A2 is highly expressed in lymph nodes, stomach, lungs, testis and skin and is moderately expressed in spleen, intestine, heart, breast and kidneys2.

SLC41A2 is reported as either over or under expressed in 75 disease states including a number of carcinomas and lymphomas according to the database on European Bioinformatics Institute (EBI).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 17/02/2021

Anti-SLC41A2 (extracellular) Antibody (#ANT-146) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC41A2 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information