Anti-SLC6A14 (extracellular) Antibody

Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14
  • New
Cat #: AGT-015
Alternative Name Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)PSEQYWNKVALQRS, corresponding to amino acid residues 213-226 of human SLC6A14 (Accession Q9UN76). 2nd extracellular loop.
        Anti-SLC6A14 (extracellular) Antibody
    Accession (Uniprot) Number Q9UN76
    Gene ID 11254
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 12/14 amino acid residues identical; rat – 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
      • Anti-SLC6A14 (extracellular) Antibody
        Western blot analysis of human MCF-7 breast adenocarcinoma cell line (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell line (lanes 2 and 4) lysates:
        1,2. Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200).
        3,4. Anti-SLC6A14 (extracellular) Antibody, preincubated with the negative control antigen.
        Anti-SLC6A14 (extracellular) Antibody
        Western blot analysis of mouse brain (lanes 1 and 3) and colon (lanes 2 and 4) lysates:
        1,2. Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200).
        3,4. Anti-SLC6A14 (extracellular) Antibody, preincubated with the negative control antigen.
        Anti-SLC6A14 (extracellular) Antibody
        Western blot analysis of rat brain lysate (lanes 1 and 3) and colon membranes (lanes 2 and 4):
        1,2. Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200).
        3,4. Anti-SLC6A14 (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-SLC6A14 (extracellular) Antibody
        Expression of SLC6A14 in mouse RTN
        Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. SLC6A14 immunoreactivity (green) is detected in reticular thalamic nucleus (RTN) cells (arrows). Cell nuclei are stained with DAPI (blue).
    References
      • SLC614A is an amino acid transporter which is a member of the Solute carrier (SLC) 6 transporter family. Currently, the SLC6 family is comprised of >300 prokaryotic and eukaryotic proteins, of which 21 have been identified in humans. SLC6 members have 12 membrane spanning domains (TM) with intracellular N- and C- termini1.

        SLC614A transports all amino acids (except glutamate and aspartate) into the cell. It has a uni-directional function and it is energized by three different driving forces: a Na+ gradient, a Cl- gradient and the membrane potential. These specific characteristics makes it important in the development of cancerous tumors2. During carcinogenesis, the developing tumor utilizes more resources than healthy tissue and, therefore, there is an over-production of transporters for glucose and amino acids. SLC614A is uniquely suited to the biological needs of tumor cells: Theoretically, this transporter has the ability to concentrate its substrates inside the cell ~1000-fold compared to the extracellular medium, which complies with the increasing demand of the tumor cell for amino acid supply2. Indeed, SLC614A has been shown to be up regulated in multiple cancer types such as, cervical cancer3, breast cancer4, and pancreatic cancer5, as well as the development of Cystic fibrosis6. Hence, the therapeutic potential in affecting the activity of this transporter is clinically significant.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Anti-SLC6A14 (extracellular) Antibody (#AGT-015) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC6A14 from rat, mouse, and human samples.

    For research purposes only, not for human use