SLC6A14 (extracellular) Blocking Peptide (#BLP-GT015) is the original antigen used for immunization during Anti-SLC6A14 (extracellular) Antibody (#AGT-015) generation. The blocking peptide binds and ‘blocks’ Anti-SLC6A14 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain (lanes 1 and 3) and colon (lanes 2 and 4) lysates:
- Western blot analysis of rat brain lysate (lanes 1 and 3) and colon membranes (lanes 2 and 4):
- Western blot analysis of human MCF-7 breast adenocarcinoma cell line (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell line (lanes 2 and 4) lysates:
- Expression of SLC6A14 in mouse RTNImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. SLC6A14 immunoreactivity (green) is detected in reticular thalamic nucleus (RTN) cells (arrows). Cell nuclei are stained with DAPI (blue).
- (C)PSEQYWNKVALQRS, corresponding to amino acid residues 213-226 of human SLC6A14 (Accession Q9UN76).