- Peptide (C)KTYFKMNYTGLAE, corresponding to amino acid residues 151-163 of rat SLC7A2 (Accession B5D5N9). 2nd extracellular loop.
- Human THP-1 monocytic leukemia cells and mouse J774 macrophage cells.
- Cell surface detection of SLC7A2 in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-SLC7A2 (extracellular)-FITC Antibody (#ANT-103-F), (5 µg).
The cationic amino acid transporter (CAT) family is comprised of four homologous membrane proteins: CAT-1, CAT-2, CAT-3 and CAT-4, encoded by the SLC7A1−4 genes, respectively. The major difference between these transporters is at the level of tissue specificity and regulation of their expression1,2.
The CAT family is included in the Na+-independent transport system, (named system y+) that mediate L-arginine uptake in most tissues and cells3.
CAT-2 (SLC7A2) is known to transport cationic amino acids such as L-arginine, L-ornithine, and L-lysine. It is considered to be the major arginine transporter in most cells and tissues. It can be expressed in two spliced variants, CAT-2A and CAT-2B. CAT-2A, is a low-affinity transporter, constitutively expressed in liver and muscle cells, whereas CAT-2B, is a high-affinity transporter, abundantly expressed in macrophages3.
Macrophages isolated from mice deficient in CAT-2 show impaired ability to transport arginine intracellularly and to produce nitric oxide in response to endotoxin, thus CAT2 may also be important in regulating immune cell function in inflammation4.
Studies have identified CAT2 as a regulator of fibrotic responses in the lung5.