Free shipping starts now, no minimum, no coupons required!

Anti-Stomatin-like Protein 1 (extracellular) Antibody

STOML1, SLP-1, STORP, Slipin-1, hUNC-24

Cat #: AIP-001
Alternative Name STOML1, SLP-1, STORP, Slipin-1, hUNC-24
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RSGYRALPLGDFDR, corresponding to amino acid residues 4-17 of mouse STOML1 (Accession Q8CI66). Extracellular, N-terminus.
Accession (Uniprot) Number Q8CI66
Gene ID 69106
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
RRID AB_2756650.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
References
  1. Mairhofer, M. et al. (2009) J. Biol. Chem. 284, 29218.
  2. Goshe, P. et al. (2010) PLoS Genet. 9, e1004063.
  3. Kozlenkov, A. et al. (2014) J. Physiol. 592, 557.
Scientific background

The Stomatin-like protein family contains five members and are characterized by the presence of a structurally conserved core called the stomatin-domain. This stomatin-domain is further related to a domain found in members of the prohibitin, flotillin and HflK/HflC protein families and has accordingly also been called the SPFH domain.

All mammalian stomatin proteins are integral membrane protein with a single, relatively short, hydrophobic membrane insertion domain, followed by the core stomatin-domain. A single conserved proline residue (known to be a potent helix breaker) in the hydrophobic region is required for the formation of the protein’s hairpin structure. The N-terminal domain is extracellular. Stomatin-like protein 1 (STOML1) has a typical stomatin-domain at the N-terminus and an additional sterol carrier protein-2 (SCP-2) domain at the C-terminus. STOML-1 is targeted to the late endosomal compartment where it interacts with stomatin. STOML-1 is predominantly expressed in the brain, in a subpopulation of sensory neurons in the dorsal root ganglia. Much lower levels are detected in other tissues1.

Following anoxia, STOML-1 expression is up-regulated by the oxidative response factor SKN-1/Nrf. SKN-1/Nrf is required for both rapid mitochondrial refusion and rapid behavioral recovery during reoxygenation suggesting that STOML-1 helps facilitate mitochondrial dynamics following anoxia2.

STOML-1 also modulates the proton-sensitive members of the acid-sensing ion channel (ASIC) family. STOML-1 strongly inhibits ASIC1a and accelerates the inactivation time constant of ASIC3, but has no effect on the splice variant ASIC1b3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-Stomatin-like Protein 1 (extracellular) Antibody (#AIP-001) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize STOML1 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information