Anti-ASIC1 Antibody

Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
Cat #: ASC-014
Alternative Name Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.
    Accession (Uniprot) Number P55926
    Gene ID 79123
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - identical; mouse - 19/20 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Recognizes isoforms ASIC1α and ASIC1β.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, wb
    May also work in: ifc
      • Western blot analysis of rat brain membranes:
        1. Anti-ASIC1 Antibody (#ASC-014), (1:200).   
        2. Anti-ASIC1 Antibody, preincubated with the control peptide antigen.
      • CHO-K1 and D54-MG transfected cells (Kapoor, N. et al. (2009) J. Biol. Chem. 284, 24526.).
      • Expression of ASIC1 in rat brain
        Immunohistochemical staining of rat globus pallidus using Anti-ASIC1 Antibody (#ASC-014). A. Parvalbumin (PV) positive neurons are shown (green). B. Neurons with typically enmeshed dendritic trees stain intensely for ASIC1 (red). C. Double labeling with mouse anti-Parvalbumin (green) reveals strong co-localization of PV with ASIC1.
      • Mouse bone marrow derived macrophage (BMMs) (Kong, X. et al. (2013) Cell. Immunol. 281, 44.).

        Human SH-SY5Y (Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.).
      • ASIC1 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC2ASIC3ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.

        There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions.  

        ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium.  

        Recently, ASIC1 has been implicated in cognitive processes such as learning and memory.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-ASIC1 Antibody
    Expression of ASIC1 in rat medulla.Immunohistochemical staining of rat brain sections using Anti-ASIC1 Antibody (#ASC-014). ASIC1 staining in the medulla is detected in the ventrolateral medulla (VLM), (left panel) and in the dorsal medulla (DM), (middle panel). Antibody specificity was demonstrated by preincubating the antibody with the control antigen (right panel).Adapted from Song, N. et al. (2016) Sci. Rep. 6, 38777. with permission of SPRINGER NATURE.

    Last update: 13/06/2019

    Anti-ASIC1 Antibody (#ASC-014) is an antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC1 from rat, human, and mouse samples, and recognizes both ASIC1 isoforms.

    For research purposes only, not for human use
    Citations
      • Western blot analysis of mouse lung lysate. Tested in ASIC1-/- mice.
        Trac, P.T. et al. (2017) Am. J. Physiol. 312, L797.
      • Mouse lung lysate. Also tested in ASIC1-/- mice.
        Trac, P.T. et al. (2017) Am. J. Physiol. 312, L797.
      • Rat DRG lysate (1:200).
        Wu, Y. et al. (2017) Cell. Mol. Neurobiol. 37, 635.
      • Mouse bone marrow-derived macrophage (BMM) lysate.
        Kong, X. et al. (2013) Cell. Immunol. 281, 44.
      • Human SH-SY5Y cell lysate.
        Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.
      • Mouse immature dendritic cells.
        Tong, J. et al. (2011) J. Immunol. 186, 3686.
      • Mouse cortex lysate (1:200).
        Hu, Z.L. et al. (2010) Am. J. Physiol. 299, C1355.
      • CHO-K1 and D54-MG transfected cell lysate.
        Kapoor, N. et al. (2009) J. Biol. Chem. 284, 24526.
      • Mouse hippocampus sections (1:200).
        Ferenczi, E.A. et al. (2016) Sci. Rep. 6, 23947.
      • Rat medulla tissue sections.
        Song, N. et al. (2016) Sci. Rep. 6, 38777.
      • Mouse primary DRGs (1:100).
        Radu, B.M. et al. (2014) Cell Biochem. Biophys. 68, 9.
      • Mouse bone marrow-derived macrophages (BMMs).
        Kong, X. et al. (2013) Cell. Immunol. 281, 44.
      • Human SH-SY5Y cell lysate.
        Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.
      • Mouse dendritic cells.
        Tong, J. et al. (2011) J. Immunol. 186, 3686.
      • Mouse cerebral cortical neurons (1:50).
        Hu, Z.L. et al. (2010) Am. J. Physiol. 299, C1355.
    Related Products
        • Guinea pig Anti-ASIC1 Antibody (#AGP-053) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #ASC-014.