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Anti-ASIC3 Antibody

Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

Cat #: ASC-018
Alternative Name Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
  • Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Accession (Uniprot) Number O35240
Gene ID 286920
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.
RRID AB_2039701.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat DRG lysate:
    Western blot analysis of rat DRG lysate:
    1. Anti-ASIC3 Antibody (#ASC-018), (1:200).
    2. Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).
  • Multiplex staining of ASIC1 and ASIC3 in rat DRG
    Multiplex staining of ASIC1 and ASIC3 in rat DRG
    Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:400) and rabbit Anti-ASIC3 Antibody (#ASC-018), (1:400). A. ASIC3 staining. B. ASIC1 staining in same section (green). C. Merge of the two images shows some co-localization (arrow). Cell nuclei were stained with DAPI (blue).
  • Rat trigeminal ganglia (1:400) (Yan, J. et al. (2013) Headache 53, 1250.).
  • Human SH-SY5Y cells (Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.)
  1. Kellenberger, S. and Schild, L. (2002) Physiol. Rev. 82, 735.
  2. Chen, C.C. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 8992.
  3. Price, M.P. et al. (2001) Neuron 32, 1071.
Scientific background

ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1ASIC2ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.

The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.

A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.

Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.

Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-ASIC3 Antibody
Expression of ASIC3 in rat trigeminal ganglia.Immunohistochemical staining of rat trigeminal ganglia (TG) sections using Anti-ASIC3 Antibody (#ASC-018). ASIC3 staining (green) appears in TG neurons (left panel) and coincides with FAST DiI staining (red), (right panel).Adapted from Meng, Q. et al. (2015) Am. J. Physiol. 309, C1. with permission of The American Physiological Society.

Last update: 08/01/2023

Anti-ASIC3 Antibody (#ASC-018) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples.

For research purposes only, not for human use



Western blot citations
  1. Rat DRG lysate (1:200).
    Wu, Y. et al. (2017) Cell. Mol. Neurobiol. 37, 635.
  2. Mouse bone marrow derived macrophage (BMM) lysate.
    Kong, X. et al. (2013) Cell. Immunol. 281, 44.
  3. Human SH-SY5Y cell lysate.
    Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.
  4. Mouse immature dentritic cells.
    Tong, J. et al. (2011) J. Immunol. 186, 3686.
Immunohistochemistry citations
  1. Rat DRG sections (1:50).
    Wu, Y. et al. (2017) Cell. Mol. Neurobiol. 37, 635.
  2. Rat trigeminal sections (1:50).
    Meng, Q. et al. (2015) Am. J. Physiol. 309, C1.
  3. Rat trigeminal ganglia (1:400).
    Yan, J. et al. (2013) Headache 53, 1250.
  4. Mouse anterial synovial sections (1:500).
    Kolker, S.J. et al. (2010) Ann. Rheum. Dis. 69, 903.
Immunocytochemistry citations
  1. Mouse primary DRGs (1:100).
    Radu, B.M. et al. (2014) Cell Biochem. Biophys. 68, 9.
  2. Human SH-SY5Y cell lysate.
    Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.
  3. Mouse dendritic cells.
    Tong, J. et al. (2011) J. Immunol. 186, 3686.
  4. Mouse synoviocytes. Also tested in ASIC3-/- mice.
    Kolker, S.J. et al. (2010) Ann. Rheum. Dis. 69, 903.
More product citations
  1. Ohbuchi, T. et al. (2010) J. Physiol. 588, 2147.
  2. Akiba, Y. et al. (2008) Gut 57, 1654.
  3. Ye, J.H. et al. (2007) Biochem. Biophys. Res. Commun. 355, 986.


Scientific Background

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