Free shipping starts now, no minimum, no coupons required!

Anti-TROY (TNFRSF19) (extracellular) Antibody

TNF receptor superfamily member 19, Toxicity and JNK inducer, TAJ, TRADE
Cat #: ANT-033
Alternative Name TNF receptor superfamily member 19, Toxicity and JNK inducer, TAJ, TRADE
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CRPHRFKEDWGFQK, corresponding to amino acid residues 75-88 of mouse TROY (Accession Q9JLL3). Extracellular, N-terminus.
Accession (Uniprot) Number Q9JLL3
Gene ID 29820
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – 13/14 amino acid residues identical.
RRID AB_2756715.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, lci, wb
May also work in: ifc*, ih*, ip*
Western blot
  • Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4):
    Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4):
    1,2. Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033), (1:400).
    3,4. Anti-TROY (TNFRSF19) (extracellular) Antibody, preincubated with TROY/TNFRSF19 (extracellular) Blocking Peptide (#BLP-NT033).
  • Western blot analysis of new born rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of new born rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1,2. Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033), (1:400).
    3,4. Anti-TROY (TNFRSF19) (extracellular) Antibody, preincubated with TROY/TNFRSF19 (extracellular) Blocking Peptide (#BLP-NT033).
Live cell imaging / Immunocytochemistry
  • Expression of TROY in human U-87 MG cells
    Expression of TROY in human U-87 MG cells
    Cell surface detection of TROY in live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033), (1:25), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.
References
  1. Locksley, R.M. et al. (2001) Cell 104, 487.
  2. Tran, N.L. et al. (2012) Neuro-Oncology 14, vi1.
  3. Paulino, V.M. et al. (2010) Mol. Cancer Res. 8, 1558.
  4. Park, J.B. et al. (2005) Neuron 45, 345.
Scientific background

Tumor necrosis factor (TNF) is a potent mediator of inflammation as well as many normal physiological functions in homeostasis and health, and anti-microbial immunity.

The TNF receptor superfamily member 19 (also known as TROY, TNFRSF19) is a type I cell surface transmembrane protein with disulfide bonds that form “cysteine-rich domains” (CRDs). These 40 amino acid pseudo-repeats are defined by 3 intra-chain disulfides generated by 6 highly conserved cysteines1. Unlike other members of the TNF receptor superfamily, TROY lacks a cytoplasmic death domain but contains a single TNF receptor-associated factor (TRAF)-binding site2. TROY is expressed on migrating or proliferating progenitor cells of the hippocampus, thalamus and cerebral cortex.

TROY is involved in the pathogenesis of various neoplasms. Glioblastoma multiforme (GBM) is the most malignant form of all primary adult brain tumors in which patient survival remains approximately 1 year. Overexpression of TROY in glioma cells activates Rac1 signaling in a Pyk2-dependent manner to drive glioma cell invasion and migration. TROY expression has been found to correlate inversely with overall patient survival in glioblastoma3.

TROY is also involved in the lack of successful axon regeneration in the adult central nervous system. Inhibition of axonal growth arises from inhibitory molecules in CNS myelin, which signal through a common receptor complex on neurons consisting of the ligand-binding Nogo-66 receptor (NgR) and two transmembrane co-receptors, p75NTR and LINGO-1. TROY can substitute p75 and form a tri-receptor complex with NgR and LINGO-1. This complex can reconstitute the activation of RhoA, a required step in the signaling pathways of myelin inhibition. Additionally, both overexpression of a truncated form of TROY lacking its intracellular domain and addition of a TROY-Fc fusion protein can block neuronal response to myelin inhibitors in a dominant-negative manner4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize TROY from human, mouse, and rat samples.

For research purposes only, not for human use
Shipping and Ordering information