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Guinea pig Anti-TRPC3 Antibody

Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Cat #: AGP-018
Alternative Name Short transient receptor potential channel 3, Transient receptor protein 3, TRP3
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1). Intracellular, C-terminus.
Accession (Uniprot) Number Q9QZC1
Gene ID 22065
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, rat - 13/14 amino acid residues identical.
RRID AB_2340963.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Guinea pig Anti-TRPC3 Antibody (#AGP-018), (1:400).
    2. Guinea pig Anti-TRPC3 Antibody preincubated with TRPC3 Blocking Peptide (#BLP-CC016).
References
  1. Roedding, A.S. et al. (2013) Brain Res. 1517, 16.
  2. Feng, S. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 11011.
  3. Kohda, K. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, E948.
  4. Lee, C.R. et al. (2013) J. Neurosci. 33, 1157.
  5. Ryu, H.J. et al. (2013) Cell. Mol. Neurobiol. 33, 575.
  6. Li, W. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109, 17087.
  7. Akita, T. et al. (2011) J. Physiol. 589, 3909.
  8. Kwan, H.Y. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 2625.
  9. Rosenberg, P. et al. (2004) Proc. Natl. Acad. Aci. U.S.A. 101, 9387.
  10. Castellano, L.E. et al. (2003) FEBS Lett. 541, 69.
  11. Trevino, C.L. et al. (2001) FEBS Lett. 509, 119.
Scientific background

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin),TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4

The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities:
1. TRPC1
2. TRPC4 and TRPC5
3. TRPC3, TRPC6, TRPC7
4. TRPC2.2,5
They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.

TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.

Different modes of activation, DAG and SOC, were described for the TRPC3 channel by different investigators working with heterologous expression systems.6,7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse TRPC3 channel. Guinea pig Anti-TRPC3 Antibody (#AGP-018) raised in guinea pig can be used in western blot analysis. It has been designed to recognize TRPC3 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPC3 Antibody (#ACC-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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