This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
This lyophilized product is shipped at room temperature. Please see its certificate of analysis for further storage instructions.
Human - will not recognize the human epitope.
3,4. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody, preincubated with the control peptide antigen.
- Montell, C. et al. (2002) Mol. Cell 9, 229.
- Clapham, D.E. (2003) Nature 426, 517.
- Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
- Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
- Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183.
- Tominaga, M. and Caterina, M.J. et al. (2004) J. Neurobiol. 61, 3.
- Voets, T. et al. (2004) Nature 430, 748.
- Pedersen, S.F. et al. (2005) Cell Calcium 38, 233.
- Muraki, K. et al. (2003) Circ. Res. 93, 829.
- Shimosato, G. et al. (2005) Pain 119, 225.
- Saunders, C.I. et al. (2007) Mol. Immunol. 44, 1429.
- Caterina, M.J. et al. (1999) Nature 398, 436.
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3
According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5
Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C).6,7 Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation.8-10 The TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway.8 TRPV2 channel is expressed in DRG neurons, different brain region and non-neuronal tissues such as spleen, lung and, intestine and in component of the immune system.5,11-12
Species reactivity key:
Immuno-colocalization of SERCA1 and TRPV2 in mouse heart.Immunohistochemical staining of mouse heart immersion-fixed, free floating frozen sections, using rabbit Anti-SERCA1 Antibody (#ACP-011) (1:200) and Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033), (1:200). A. SERCA1 staining (green) appears in T tubules (arrows). B. TRPV2 staining (red) in same section is detected in intercalated discs (diagonal arrows) and T tubules (horizontal arrow). C. Merge of panels A and B demonstrates co-localization of SERCA1 and TRPV2. Nuclei are stained using DAPI (blue).
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TRPV2. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033) raised in guinea pigs, can be used in western blot and immunocytochemistry. It has been designed to recognize TRPV2 from mouse and rat samples. The antigen used to immunize guinea pigs is the same as Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.
Products for control experiments
- Anti-TRPV2 (VRL1) Antibody (#ACC-032). This antibody recognizes a different epitope and thus can also be used as a control for detecting TRPV2.
Antibodies for live cell flow cytometry experiments
- Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody (#ACC-039-F). This FITC-conjugated antibody can be used to detect TRPV2 in live cell flow cytometry.
Antibodies for live cell imaging experiments
- Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039). This antibody recognizes an extracellular epitope and can be used to detect TRPV2 in live cells. This antibody has been raised against the same epitope as #AGP-033.
- Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody (#ACC-039-F). A fluorescent labeled primary antibody. It can be used to detect TRPV2 in live cells.