Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody

Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B
    Cat #: AGP-033
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: r
    May also work in: m
    Immunogen
    Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2). 1st extracellular loop.
    Accession (Uniprot) Number Q9WUD2
    Gene ID 29465
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 15/16.
    Human - will not recognize the human epitope.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Will not recognize the human epitope.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain (lanes 1 and 3) and RBL cell lysate (lanes 2 and 4):
    1,2. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033), (1:2000).
    3,4. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Immuno-colocalization of TRPV2 and mGluR5 in rat DRG
    Immunohistochemistry of rat dorsal root ganglion using Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033) (1:60) and Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR), (red), (1:60). A. TRPV2 staining (green). B. mGluR5 staining (red). C. Merge of A and B demonstrates co-localization of TRPV2 and mGluR5 in DRG cells. Nuclei are stained using DAPI as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    Expression of TRPV2 in rat RBL cells
    Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033), (1:100) followed by goat anti-guinea pig-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge image of A and B.
    References
    1. Montell, C. et al. (2002) Mol. Cell 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev55, 591.
    5. Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci23, 183.
    6. Tominaga, M. and Caterina, M.J. et al. (2004) J. Neurobiol61, 3.
    7. Voets, T. et al. (2004) Nature 430, 748.
    8. Pedersen, S.F. et al. (2005) Cell Calcium 38, 233.
    9. Muraki, K. et al. (2003) Circ. Res93, 829.
    10. Shimosato, G. et al. (2005) Pain 119, 225.
    11. Saunders, C.I. et al. (2007) Mol. Immunol44, 1429.
    12. Caterina, M.J. et al. (1999) Nature 398, 436.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3

    According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

    Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C).6,7 Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation.8-10 The TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway.8 TRPV2 channel is expressed in DRG neurons, different brain region and non-neuronal tissues such as spleen, lung and, intestine and in component of the immune system.5,11-12

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody
    Immuno-colocalization of SERCA1 and TRPV2 in mouse heart.Immunohistochemical staining of mouse heart immersion-fixed, free floating frozen sections, using rabbit Anti-SERCA1 Antibody (#ACP-011) (1:200) and Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033), (1:200). A. SERCA1 staining (green) appears in T tubules (arrows). B. TRPV2 staining (red) in same section is detected in intercalated discs (diagonal arrows) and T tubules (horizontal arrow). C. Merge of panels A and B demonstrates co-localization of SERCA1 and TRPV2. Nuclei are stained using DAPI (blue).
    Last update: 06/12/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TRPV2. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033) raised in guinea pigs, can be used in western blot and immunocytochemistry. It has been designed to recognize TRPV2 from mouse and rat samples. The antigen used to immunize guinea pigs is the same as Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use
    Citations
    Related Products
      • Products for control experiments
        1. Anti-TRPV2 (VRL1) Antibody (#ACC-032). This antibody recognizes a different epitope and thus can also be used as a control for detecting TRPV2.