Aquaporin 1 Blocking Peptide (#BLP-QP001) is the original antigen used for immunization during Anti-Aquaporin 1 Antibody (#AQP-001) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney membranes:
- Expression of AQP1 in rat kidneyImmunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1 Antibody (#AQP-001), (1:100). AQP1 staining (brown) is present in both distal (DT) and proximal (PT) convoluted tubules in the renal cortex. Note that a weak staining is also present in collecting ducts (CD) while glomeruli (Gl) are negative. Hematoxilin is used as the counterstain.
- Mouse paraffin-embedded kidney sections (1:100) (Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283.).
- (C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human AQP1 (Accession P29972).