Anti-Aquaporin 1 Antibody

AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule
    Cat #: AQP-001
    Alternative Name AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human AQP1 (Accession P29972). Intracellular, C-terminus.
    • Anti-Aquaporin 1 Antibody
    Accession (Uniprot) Number P29972
    Gene ID 358
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, cattle, sheep - identical; dog - 18/19 amino acid residues identical; frog (Rana esculenta), toad (Bufo marinus) - 14/19 amino acid residues identical.
    RRID AB_2039726.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-Aquaporin 1 Antibody
      Western blot analysis of rat kidney membranes:
      1. Anti-Aquaporin 1 Antibody (AQP-001), (1:400).
      2. Anti-Aquaporin 1 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Aquaporin 1 Antibody
      Expression of AQP1 in rat kidney
      Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1 Antibody (#AQP-001), (1:100). AQP1 staining (brown) is present in both distal (DT) and proximal (PT) convoluted tubules in the renal cortex. Note that a weak staining is also present in collecting ducts (CD) while glomeruli (Gl) are negative. Hematoxilin is used as the counterstain.
    • Mouse paraffin-embedded kidney sections (1:100) (Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283.).
    References
    1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
    2. Ma, T. et al. (1998) J Biol Chem 273, 4296.
    3. Hu. J. and Verkman, A.S. (2006) FASEB J. 20, 1892.
    Scientific background

    Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

    The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2AQP-4 and AQP-5 belongs to the first group1.

    Little is known about the function of the two newest members, AQP-11 and AQP-12.

    The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.

    AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration2. In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:


    Knockout validation of Anti-Aquaporin 1 Antibody in mouse platelets.Western blot analysis of mouse platelets lysates using Anti-Aquaporin 1 Antibody (#AQP-001) (lower panel). AQP1 immunodetection is observe in platelets from wild type mice (+/+) but absent in platelets from AQP1-/- mice (-/-). GAPDH (upper panel) is used as the loading control.Adapted from Agbani, E.O. et al. (2018) JCI Insight 3, e99062. with permission of the American Society for Clinical Investigation.

    Last update: 24/01/2020

    Anti-Aquaporin 1 Antibody (#AQP-001) is a highly specific antibody directed against an epitope of the human AQP1 channel. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP1 from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Published figures using this product
    • Anti-Aquaporin 1 Antibody
      Expression of Aquaporin 1 in mouse kidney
      Immunohistochemical staining of mouse kidney sections using Anti-Aquaporin 1 Antibody (#AQP-001). AQP1 staining (red) along the thin limb of Henle cells does not change following ischemia.
      Adapted from Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283. with permission of The American Physiological Society.
    KO validation citations
    1. Mouse platelet lysate. Tested in AQP1-/- mice.
      Agbani, E.O. et al. (2018) JCI Insight 3, e99062.
    Western blot citations
    1. Mouse platelet lysate. Tested in AQP1-/- mice.
      Agbani, E.O. et al. (2018) JCI Insight 3, e99062.
    2. Rat kidney lysate.
      Chung, S. et al. (2019) Front. Physiol. 10, 271.
    Immunohistochemistry citations
    1. Rat kidney sections.
      Chung, S. et al. (2019) Front. Physiol. 10, 271.
    2. Mouse kidney sections.
      Kong, M.J. et al. (2019) Redox Biol. 20, 38.
    3. Mouse kidney sections.
      Han, S.J. et al. (2016) J. Am. Soc. Nephrol. 28, 1200.
    4. Mouse paraffin-embedded kidney sections (1:100).
      Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283.
    More product citations
    1. Ikarashi, N. et al. (2011) Am. J. Physiol. 301, G887.
    2. Kim, J. et al. (2011) Anat. Cell Biol. 44, 186.
    3. Kim, J. et al. (2010) Am. J. Physiol. 298, F1118.
    4. Park, J.W. et al. (2010) Am. J. Physiol. 298, F301.
    5. Satake, M. et al. (2010) Biol. Pharm. Bull. 33, 1965.
    6. Bae, E.H. et al. (2009) Nephrol. Dial. Transplant. 24, 2692.
    7. Kim, J. et al. (2009) Am. J. Physiol. 296, F622.
    8. Allory, Y. et al. (2008) Kidney Int. 73, 751.
    9. Lodewyck, D. et al. (2007) Arch Otolaryngol Head Neck Surg. 133, 557.
    10. Watson, K.J. et al. (2007) Chem. Senses. 32, 411.
    11. Lee-Kwon, W. et al. (2005) Am. J. Physiol. 288, C942.
    12. Mulder, J. et al. (2005) Am. J. Physiol. 288, R1417.
    13. Yeum, C.H. et al. (2003) Scand. J. Urol. Nephrol. 37, 99.
    14. Yoshino, J. et al. (2003) J. Am. Soc. Nephrol. 14, 3090.
    15. Kim, S.W. et al. (2001) J. Am. Soc. Nephrol. 12, 875.
    16. Kim, S.W. et al. (2001) J. Am. Soc. Nephrol. 12, 2019.
    17. Markert, J.M. et al. (2001) Physiol. Genomics 5, 21.
    18. Patil, R.V. et al. (2001) Am. J. Physiol. 281, C1139.
    19. Quigley, R. et al. (1998) J. Membr. Biol. 164, 177.
    Shipping and Ordering information