Aquaporin 3 Blocking Peptide (#BLP-QP003) is the original antigen used for immunization during Anti-Aquaporin 3 Antibody (#AQP-003) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney membranes:
- Expression of Aquaporin 3 in rat colonImmunohistochemical staining of paraffin embedded longitudinal section of rat colon using Anti-Aquaporin 3 Antibody (#AQP-003), (brown). AQP3 is detected in absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Hematoxilin is used as the counterstain. Mucosa (M), submucosa (SM) and muscularis externa (ME).
- (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3 (Accession P47862).