Anti-Aquaporin 3 Antibody

AQP3, Aquaglyceroporin-3, GIL, GLIP
    Cat #: AQP-003
    Alternative Name AQP3, Aquaglyceroporin-3, GIL, GLIP
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3 (Accession P47862). Intracellular, C-terminus.
        Anti-Aquaporin 3 Antibody
    Accession (Uniprot) Number P47862
    Gene ID 65133
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 17/18 amino acid residues identical; sheep, human - 15/18 amino acid residues identical.
    RRID AB_2039731.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.4 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, ip, wb
    May also work in: ic*
      • Anti-Aquaporin 3 Antibody
        Western blot analysis of rat kidney membranes:
        1. Anti-Aquaporin 3 Antibody (#AQP-003), (1:200).
        2. Anti-Aquaporin 3 Antibody, preincubated with the negative control antigen.
      • Anti-Aquaporin 3 Antibody
        Expression of Aquaporin 3 in rat colon
        Immunohistochemical staining of paraffin embedded longitudinal section of rat colon using Anti-Aquaporin 3 Antibody (#AQP-003), (brown). AQP3 is detected in absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Hematoxilin is used as the counterstain. Mucosa (M), submucosa (SM) and muscularis externa (ME).
      • Human airway epithelial cell (Avril-Delplanque, A. et al. (2005) Stem Cells 23, 992.).
        The negative control antigen is not suitable for this application.
    References
      • Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow the passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

        The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7AQP-9 and AQP-10.1 Little is known about the function of the two newest members, AQP-11 and AQP-12.  

        The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1

        AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.2 Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Aquaporin 3 Antibody
    Expression of AQP3 in mouse colon.
    Immunohistochemical staining of mouse colon sections using Anti-Aquaporin 3 Antibody (#AQP-003). AQP3 staining (red) is detected in mouse distal colon, and co-localizes with e-cadherin (green). AQP3 is not detected in AQP3-/- cells.Adapted from Thiagarajah, J.R. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 568. with permission of the National Academy of Sciences.
    Last update: 24/01/2020

    Anti-Aquaporin 3 Antibody (#AQP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize the AQP3 channel from rat, human, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Immunohistochemical staining of mouse colon sections. Also tested in AQP3-/- tissue sections.
        Thiagarajah, J.R. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 568.
      • Rat kidney lysate.
        Chung, S. et al. (2019) Front. Physiol. 10, 271.
      • Rat digestive system lysate (1:400).
        Zhao, G. et al. (2016) Biotech. Histochem. 91, 269.
      • Rat colon lysate.
        Kon, R. et al. (2015) Toxicol. Sci. 145, 337.
      • Rat colon and ileum lysates (1:400).
        Zhao, G. et al. (2014) Biochem. Biophys. Res. Commun. 443, 161.
      • Rat kidney sections.
        Chung, S. et al. (2019) Front. Physiol. 10, 271.
      • Mouse colon sections. Also tested in AQP3-/- tissue sections.
        Thiagarajah, J.R. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 568.
      • Sheep ovary sections (1:1000).
        Sales, A.D. et al. (2016) Cell Tissue Res. 365, 415.
      • Rat digestive system sections (1:100).
        Zhao, G. et al. (2016) Biotech. Histochem. 91, 269.
      • Rat colon sections.
        Kon, R. et al. (2015) Toxicol. Sci. 145, 337.
      • Rat colon and ileum sections (1:100).
        Zhao, G. et al. (2014) Biochem. Biophys. Res. Commun. 443, 161.
      • Ikarashi, N. et al. (2011) Biol. Pharm. Bull. 34, 238.
      • Satake, M. et al. (2010) Biol. Pharm. Bull. 33, 1965.
      • Allory, Y. et al. (2008) Kidney Int. 73, 751.
      • Bae, E.H. et al. (2008) Am. J. Physiol. Renal Physiol. 294, F272.
      • Ma, S.K. et al. (2007) Kidney Blood Press. Res. 30, 8.
      • Leung, J.C. et al. (2005) Nephrology 10, 63.
      • Edashige, K. et al. (2003) Biol. Reprod. 68, 87.
      • Yeum, C.H. et al. (2003) Scand. J. Urol. Nephrol. 37, 99.
      • Kim, S.W. et al. (2001) J. Am. Soc. Nephrol. 12, 875.
      • Kim, S.W. et al. (2001) J. Am. Soc. Nephrol. 12, 2019.
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