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Anti-Aquaporin 3 Antibody

AQP3, Aquaglyceroporin-3, GIL, GLIP

Cat #: AQP-003
Alternative Name AQP3, Aquaglyceroporin-3, GIL, GLIP
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3 (Accession P47862). Intracellular, C-terminus.
    Accession (Uniprot) Number P47862
    Gene ID 65133
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 17/18 amino acid residues identical; sheep, human - 15/18 amino acid residues identical.
    RRID AB_2039731.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.4 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, ip, wb
    May also work in: ic*
    Western blot
    • Western blot analysis of rat kidney membranes:
      Western blot analysis of rat kidney membranes:
      1. Anti-Aquaporin 3 Antibody (#AQP-003), (1:200).
      2. Anti-Aquaporin 3 Antibody, preincubated with Aquaporin 3 Blocking Peptide (#BLP-QP003).
    Immunoprecipitation
    Immunohistochemistry
    • Expression of Aquaporin 3 in rat colon
      Expression of Aquaporin 3 in rat colon
      Immunohistochemical staining of paraffin embedded longitudinal section of rat colon using Anti-Aquaporin 3 Antibody (#AQP-003), (brown). AQP3 is detected in absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Hematoxilin is used as the counterstain. Mucosa (M), submucosa (SM) and muscularis externa (ME).
    Indirect flow cytometry
    • Human airway epithelial cell (Avril-Delplanque, A. et al. (2005) Stem Cells 23, 992.).
    • The blocking peptide is not suitable for this application.
    References
    1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
    2. Hara, M. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 7360.
    3. Hara-Chikuma, M. and Verkman, A.S. (2008) Mol. Cell. Biol. 28, 326.
    Scientific background

    Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow the passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

    The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7AQP-9 and AQP-10.1 Little is known about the function of the two newest members, AQP-11 and AQP-12.  

    The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1

    AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.2 Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Aquaporin 3 Antibody
    Expression of AQP3 in mouse colon.
    Immunohistochemical staining of mouse colon sections using Anti-Aquaporin 3 Antibody (#AQP-003). AQP3 staining (red) is detected in mouse distal colon, and co-localizes with e-cadherin (green). AQP3 is not detected in AQP3-/- cells.Adapted from Thiagarajah, J.R. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 568. with permission of the National Academy of Sciences.
    Last update: 18/10/2021

    Anti-Aquaporin 3 Antibody (#AQP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize the AQP3 channel from rat, human, and mouse samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Immunohistochemical staining of mouse colon sections. Also tested in AQP3-/- tissue sections.
      Thiagarajah, J.R. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 568.
    Western blot citations
    1. Rat kidney lysate.
      Chung, S. et al. (2019) Front. Physiol. 10, 271.
    2. Rat digestive system lysate (1:400).
      Zhao, G. et al. (2016) Biotech. Histochem. 91, 269.
    3. Rat colon lysate.
      Kon, R. et al. (2015) Toxicol. Sci. 145, 337.
    4. Rat colon and ileum lysates (1:400).
      Zhao, G. et al. (2014) Biochem. Biophys. Res. Commun. 443, 161.
    Immunoprecipitation citations
    1. Mouse epidermal keratinocytes (1 μg per ml).
      Zheng, X. and Bollinger Bollag, W. et al. (2003) J. Invest. Dermatol. 121, 1487.
    Immunohistochemistry citations
    1. Rat kidney sections.
      Chung, S. et al. (2019) Front. Physiol. 10, 271.
    2. Mouse colon sections. Also tested in AQP3-/- tissue sections.
      Thiagarajah, J.R. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 568.
    3. Sheep ovary sections (1:1000).
      Sales, A.D. et al. (2016) Cell Tissue Res. 365, 415.
    4. Rat digestive system sections (1:100).
      Zhao, G. et al. (2016) Biotech. Histochem. 91, 269.
    5. Rat colon sections.
      Kon, R. et al. (2015) Toxicol. Sci. 145, 337.
    6. Rat colon and ileum sections (1:100).
      Zhao, G. et al. (2014) Biochem. Biophys. Res. Commun. 443, 161.
    Indirect flow cytometry citations
    1. Human airway epithelial cell.
      Avril-Delplanque, A. et al. (2005) Stem Cells 23, 992.
    More product citations
    1. Ikarashi, N. et al. (2011) Biol. Pharm. Bull. 34, 238.
    2. Satake, M. et al. (2010) Biol. Pharm. Bull. 33, 1965.
    3. Allory, Y. et al. (2008) Kidney Int. 73, 751.
    4. Bae, E.H. et al. (2008) Am. J. Physiol. Renal Physiol. 294, F272.
    5. Ma, S.K. et al. (2007) Kidney Blood Press. Res. 30, 8.
    6. Leung, J.C. et al. (2005) Nephrology 10, 63.
    7. Edashige, K. et al. (2003) Biol. Reprod. 68, 87.
    8. Yeum, C.H. et al. (2003) Scand. J. Urol. Nephrol. 37, 99.
    9. Kim, S.W. et al. (2001) J. Am. Soc. Nephrol. 12, 875.
    10. Kim, S.W. et al. (2001) J. Am. Soc. Nephrol. 12, 2019.

    Specifications

    Scientific Background

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