Aquaporin 5 Blocking Peptide (#BLP-QP005) is the original antigen used for immunization during Anti-Aquaporin 5 Antibody (#AQP-005) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse salivary gland (1 and 3) and rat lung (2 and 4):
- Expression of AQP5 in rat lungImmunohistochemical staining of rat lung sections using Anti-Aquaporin 5 Antibody (#AQP-005), (left panel). The strong staining in the alveoli (red brown) is consistent with type 1 pneumocytes (arrows). Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
- (C)DHREERKKTIELTAH, corresponding to amino acid residues 251-265 of rat AQP5 (Accession P47864).