Anti-Aquaporin 5 Antibody

AQP5
    Cat #: AQP-005
    Alternative Name AQP5
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)DHREERKKTIELTAH corresponding to amino acid residues 251-265 of rat AQP5 (Accession number P47864). Intracellular, C-terminus.
        Anti-Aquaporin 5 Antibody
    Accession (Uniprot) Number P47864
    Gene ID 25241
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 10/16 amino acid residues identical.
    RRID AB_2039736.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.4 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-Aquaporin 5 Antibody
        Western blot analysis of mouse salivary gland (1 and 3) and rat lung (2 and 4):
        1,2. Anti-Aquaporin 5 Antibody (#AQP-005), (1:200).
        3,4. Anti-Aquaporin 5 Antibody, preincubated with the control peptide antigen.
        Human primary lung epithelial cells (Marconett, C.N. et al. (2013) PLoS Genet. 9, e1003513.).
      • Anti-Aquaporin 5 Antibody
        Expression of AQP5 in rat lung
        Immunohistochemical staining of rat lung sections using Anti-Aquaporin 5 Antibody (#AQP-005), (left panel). The strong staining in the alveoli (red brown) is consistent with type 1 pneumocytes (arrows). Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
      • Mouse submandibular salivary cells (1:400) (Joo, E.E. et al. (2016) J. Dent. Res. 95, 1518.).
    References
    • 1. Agre, P. et al. (2002) J. Physiol. 542.1, 3.
    • 2. Towne, J.E. et al. (2000) Am. J. Respir. Cell Mol. Biol. 22, 34.
    • 3. Yang, F. et al. (2002) J. Biol. Chem. 278, 32173.
      • The importance of the aquaporin water channels was underscored by awarding the 2003 Nobel Prize in Chemistry to Peter Agre “for the discovery of water channels”. Today, eleven mammalian proteins that belong to the aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. 

        Aquaporin 5 (AQP5) is expressed in secretory glands, lung and eye, where it works in modulating the rate of fluid release. Since a number of pathological conditions including pulmonary edema, respiratory distress syndrome and congestive heart failure are characterized by disrupted fluid transport, it has been suggested that modulation of the expression of AQP5 could be a novel modality of treatment of the above illnesses. Indeed, several studies have shown that AQP5 expression is downregulated in animal models of acute lung infection.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Aquaporin 5 Antibody
    Expression of Aquaporin 5 in mouse ATI-like cells.Immunocytochemical staining of mouse ATI-like cells isolated from lungs using Anti-Aquaporin 5 Antibody (#AQP-005). a. AQP5 staining (green). b. AQP5 staining is abolished when antibody is preincubated with the control antigen. c. Cells incubated with a matching isotype control. d. A negative control using mouse endothelial cells.Adapted from Barnthaler, T. et al. (2017) Sci. Rep. 7, 7923. with permission of Nature publishing group.
    Last update: 24/01/2020

    Anti-Aquaporin 5 Antibody (#AQP-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AQP5 from mouse, rat, and human samples.

    For research purposes only, not for human use
    Citations
      • Rat AT2 cell lysate.
        Liebler, J.M. et al. (2016) Am. J. Physiol. 310, L114.
      • Human primary lung epithelial cells.
        Marconett, C.N. et al. (2013) PLoS Genet. 9, e1003513.
      • Mouse submandibular glands (1:400).
        Joo, E.E. et al. (2016) J. Dent. Res. 95, 1518.
      • Mouse submandibular salivary glands.
        Gervais, E.M. et al. (2015) Anat. Rec. 298, 1622.
      • Rat duodenum.
        Collaco, A.M. et al. (2013) Am. J. Physiol. 305, G258.
      • Mouse ATI-like cells.
        Barnthaler, T. et al. (2017) Sci. Rep. 7, 7923.
      • Mouse submandibular salivary cells (1:400).
        Joo, E.E. et al. (2016) J. Dent. Res. 95, 1518.
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