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Anti-Aquaporin 5 Antibody

AQP5
Cat #: AQP-005
Alternative Name AQP5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DHREERKKTIELTAH corresponding to amino acid residues 251-265 of rat AQP5 (Accession number P47864). Intracellular, C-terminus.
Accession (Uniprot) Number P47864
Gene ID 25241
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 10/16 amino acid residues identical.
RRID AB_2039736.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.4 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of mouse salivary gland (1 and 3) and rat lung (2 and 4):
    Western blot analysis of mouse salivary gland (1 and 3) and rat lung (2 and 4):
    1,2. Anti-Aquaporin 5 Antibody (#AQP-005), (1:200).
    3,4. Anti-Aquaporin 5 Antibody, preincubated with Aquaporin 5 Blocking Peptide (#BLP-QP005).
  • Human primary lung epithelial cells (Marconett, C.N. et al. (2013) PLoS Genet. 9, e1003513.).
Immunohistochemistry
  • Expression of AQP5 in rat lung
    Expression of AQP5 in rat lung
    Immunohistochemical staining of rat lung sections using Anti-Aquaporin 5 Antibody (#AQP-005), (left panel). The strong staining in the alveoli (red brown) is consistent with type 1 pneumocytes (arrows). Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
Immunocytochemistry
  • Mouse submandibular salivary cells (1:400) (Joo, E.E. et al. (2016) J. Dent. Res. 95, 1518.).
References
  1. Agre, P. et al. (2002) J. Physiol. 542.1, 3.
  2. Towne, J.E. et al. (2000) Am. J. Respir. Cell Mol. Biol. 22, 34.
  3. Yang, F. et al. (2002) J. Biol. Chem. 278, 32173.
Scientific background

The importance of the aquaporin water channels was underscored by awarding the 2003 Nobel Prize in Chemistry to Peter Agre “for the discovery of water channels”. Today, eleven mammalian proteins that belong to the aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. 

Aquaporin 5 (AQP5) is expressed in secretory glands, lung and eye, where it works in modulating the rate of fluid release. Since a number of pathological conditions including pulmonary edema, respiratory distress syndrome and congestive heart failure are characterized by disrupted fluid transport, it has been suggested that modulation of the expression of AQP5 could be a novel modality of treatment of the above illnesses. Indeed, several studies have shown that AQP5 expression is downregulated in animal models of acute lung infection.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-Aquaporin 5 Antibody
Expression of Aquaporin 5 in mouse ATI-like cells.Immunocytochemical staining of mouse ATI-like cells isolated from lungs using Anti-Aquaporin 5 Antibody (#AQP-005). a. AQP5 staining (green). b. AQP5 staining is abolished when antibody is preincubated with the control antigen. c. Cells incubated with a matching isotype control. d. A negative control using mouse endothelial cells.Adapted from Barnthaler, T. et al. (2017) Sci. Rep. 7, 7923. with permission of Nature publishing group.
Last update: 16/08/2020

Anti-Aquaporin 5 Antibody (#AQP-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AQP5 from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat AT2 cell lysate.
    Liebler, J.M. et al. (2016) Am. J. Physiol. 310, L114.
  2. Human primary lung epithelial cells.
    Marconett, C.N. et al. (2013) PLoS Genet. 9, e1003513.
Immunohistochemistry citations
  1. Mouse submandibular glands (1:400).
    Joo, E.E. et al. (2016) J. Dent. Res. 95, 1518.
  2. Mouse submandibular salivary glands.
    Gervais, E.M. et al. (2015) Anat. Rec. 298, 1622.
  3. Rat duodenum.
    Collaco, A.M. et al. (2013) Am. J. Physiol. 305, G258.
Immunocytochemistry citations
  1. Mouse ATI-like cells.
    Barnthaler, T. et al. (2017) Sci. Rep. 7, 7923.
  2. Mouse submandibular salivary cells (1:400).
    Joo, E.E. et al. (2016) J. Dent. Res. 95, 1518.
More product citations
  1. Hollborn, M. et al. (2011) Curr. Eye Res. 36, 850.
  2. Demaio, L. et al. (2009) Am. J. Physiol. 296, L1051.
  3. Yamazaki, D. et al. (2009) Development 136, 2355.
  4. Nagai, K. et al. (2007) Biochem. Biophys. Res. Com. 354, 579.
  5. Takayasu, H. et al. (2007) J. Ped. Surg. 42, 415.
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