Aquaporin 9 Blocking Peptide (#BLP-QP009) is the original antigen used for immunization during Anti-Aquaporin 9 Antibody (#AQP-009) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 9 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes:
- Western blot analysis of rat H4-II-E-C3 hepatoma cell line lysate:
- Western blot analysis of mouse brain membranes:
- Expression of AQP9 in rat liverImmunohistochemical staining of rat liver paraffin embedded sections using Anti-Aquaporin 9 Antibody (#AQP-009) (1:100). AQP9 (brown) is expressed in hepatocytes of the liver parenchyma. Hematoxilin is used as the counterstain.
- Expression of AQP9 in rat epididymisImmunohistochemical staining of rat testis paraffin embedded section using Anti-Aquaporin 9 Antibody (#AQP-009), (1:100). AQP9 (brown) is expressed in the columnar epithelium of the epididymus (arrows). Hematoxilin is used as the counterstain.
- (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP9 (Accession P56627).