ASIC2a Blocking Peptide (#BLP-SC012) is the original antigen used for immunization during Anti-ASIC2a Antibody (#ASC-012) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC2a primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Rat DRG sections (Alvarez de la Rosa, D. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 2326.).
- Expression of ASIC2a in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-ASIC2a Antibody (#ASC-012), (1:300), followed by donkey anti-rabbit-Cy3. A. ASIC2a immunoreactivity (red) appeared in neuronal soma (horizontal arrow) in the ventral horn of the spinal cord. B. Pre-incubation of the antibody with ASIC2a Blocking Peptide (BLP-SC012), suppressed staining. Cell nuclei are stained with DAPI (blue).
- DLKESPSEGSLQPSSIQC, corresponding to amino acid residues 2-18 of human ASIC2a (Accession Q16515).