ASTN1 (extracellular) Blocking Peptide (#BLP-NR151) is the original antigen used for immunization during Anti-ASTN1 (extracellular) Antibody (#ANR-151) generation. The blocking peptide binds and ‘blocks’ Anti-ASTN1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Expression of Astrotactin-1 in mouse hippocampus and cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Astrotactin-1 immunoreactivity (green) is observed in neurons (arrows) of hippocampal CA3 pyramidal layer (P). B. Astrotactin-1 staining (green) appears in neurons of cortex (arrows).
- (C)RQEKVTDRMDHSK, corresponding to amino acid residues 1068-1080 of mouse Astrotactin-1 (Accession Q61137).