- Peptide (C)RQEKVTDRMDHSK, corresponding to amino acid residues 1068-1080 of mouse Astrotactin-1 (Accession Q61137). Extracellular, C-terminus.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200).
3,4. Anti-ASTN1 (extracellular) Antibody, preincubated with ASTN1 (extracellular) Blocking Peptide (#BLP-NR151).
- Expression of Astrotactin-1 in mouse hippocampus and cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Astrotactin-1 immunoreactivity (green) is observed in neurons (arrows) of hippocampal CA3 pyramidal layer (P). B. Astrotactin-1 staining (green) appears in neurons of cortex (arrows).
Astrotactin 1 (ASTN-1) is an integral membrane perforin-like protein known to play a critical role in neurodevelopment. The protein is responsible for the formation of neuron–glial fiber contacts in the cerebellum where it plays an important role in neuronal migration1,2.
Astrotactin 1 is also expressed in post-mitotic neuronal precursors of the cerebellum, hippocampus, cerebrum and olfactory bulb. The protein demonstrates an active role in the establishment of laminar structures1.
Astrotactin 1 structure reveals a large extracellular C-terminal domain that is complemented by an N-terminal cytosolic domain separated by two transmembrane α-helices1.
Studies have found that genetic variations associated with Astrotactin 1 are connected to various neurodevelopmental disorders and other neurological pathologies including Alzheimer's disease1. In addition, ASTN1 null mice exhibit slow migration rates of cerebellar granule cells, smaller cerebellar size and reduced glial neuron binding2 and poorer balance and coordination than wild-type1. Conversely, increased levels of Astrotactin 1 are detected in rat brain following hippocampal injury, linking the protein to repair processes.
Species reactivity key:
Anti-ASTN1 (extracellular) Antibody (#ANR-151) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Astrotactin-1 from rat, mouse, and human samples.