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Anti-ASTN1 (extracellular) Antibody

Astrotactin-1

Cat #: ANR-151
Alternative Name Astrotactin-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RQEKVTDRMDHSK, corresponding to amino acid residues 1068-1080 of mouse Astrotactin-1 (Accession Q61137). Extracellular, C-terminus.
Accession (Uniprot) Number Q61137
Gene ID 11899
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
RRID AB_2756708.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1,2. Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200).
    3,4. Anti-ASTN1 (extracellular) Antibody, preincubated with ASTN1 (extracellular) Blocking Peptide (#BLP-NR151).
Immunohistochemistry
  • Expression of Astrotactin-1 in mouse hippocampus and cortex
    Expression of Astrotactin-1 in mouse hippocampus and cortex
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Astrotactin-1 immunoreactivity (green) is observed in neurons (arrows) of hippocampal CA3 pyramidal layer (P). B. Astrotactin-1 staining (green) appears in neurons of cortex (arrows).
References
  1. Ni, T. et al. (2016) Open Biol. 6, 160053.
  2. Reiner, O. et al. (2016) J. Neurochem. 136, 440.
Scientific background

Astrotactin 1 (ASTN-1) is an integral membrane perforin-like protein known to play a critical role in neurodevelopment. The protein is responsible for the formation of neuron–glial fiber contacts in the cerebellum where it plays an important role in neuronal migration1,2.

Astrotactin 1 is also expressed in post-mitotic neuronal precursors of the cerebellum, hippocampus, cerebrum and olfactory bulb. The protein demonstrates an active role in the establishment of laminar structures1.

Astrotactin 1 structure reveals a large extracellular C-terminal domain that is complemented by an N-terminal cytosolic domain separated by two transmembrane α-helices1.

Studies have found that genetic variations associated with Astrotactin 1 are connected to various neurodevelopmental disorders and other neurological pathologies including Alzheimer's disease1. In addition, ASTN1 null mice exhibit slow migration rates of cerebellar granule cells, smaller cerebellar size and reduced glial neuron binding2 and poorer balance and coordination than wild-type1. Conversely, increased levels of Astrotactin 1 are detected in rat brain following hippocampal injury, linking the protein to repair processes.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-ASTN1 (extracellular) Antibody (#ANR-151) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Astrotactin-1 from rat, mouse, and human samples.

For research purposes only, not for human use
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