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Waglerin-1

Waglerin-I, Wtx-1, Lethal peptide I

A Potent Antagonist of nAChR and a Modulator of GABA(A) Receptors

Cat #: STW-001
Alternative Name Waglerin-I, Wtx-1, Lethal peptide I
Lyophilized Powder yes
  • Bioassay Tested
    • Origin Synthetic peptide
    MW: 2520 Da
    Purity: >99% (HPLC)
    Effective concentration 1- 5 µM.
    Sequence GGKPDLRPCHPPCHYIPRPKPR.
    Modifications Disulfide bond between Cys9-Cys13.
    Structure
    Molecular formula C112H175N37O26S2.
    Activity Waglerin-1 blocks ε subunit of muscle adult nAChR and modulates rodent GABA(A)/GABRA1 receptors1,2.
    References-Activity
    1. McArdle, J.J. et al. (1999) J. Pharmacol. Exp. Ther. 289, 543.
    2. Molles, B.E. et al. (2002) J. Biol. Chem. 277, 5433
    Accession number P24335.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Waglerin-I inhibits muscle embryonic α1/β1/γ/δ nAChR currents heterologously expressed in Xenopus oocytes.
      Alomone Labs Waglerin-I inhibits muscle embryonic α1/β1/γ/δ nAChR currents heterologously expressed in Xenopus oocytes.
      A. Time course of Waglerin-1 (#STW-001) action on muscle α1/β1/γ/δ nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at -80 mV and oocytes were stimulated by exposure to 10 μM acetylcholine every 100 seconds. 10 µM (for 3 min) Waglerin-1 were perfused during the period marked by the bar, as indicated. B. Superimposed traces of muscle nAChR channel current in the absence (black) and presence (green) of 10 µM Waglerin-1 (taken from the experiment in A).
    References - Scientific background
    1. Sellin, L.C. et al. (1996) Biophys. J. 70, 3.
    2. McArdle, J.J. et al. (1999) J. Pharmacol. Exp. Ther. 289, 543.
    3. Molles, B.E. et al. (2002) J. Biol. Chem. 277, 5433.
    4. Ye, J.H. and McArdle, J.J. (1997) J. Pharmacol. Exp. Ther. 282, 74.
    Scientific background

    Waglerin-1 is a 22 amino acid peptide toxin isolated from the Southeast Asian snake Trimeresurus wagleri1. Two other similar peptides named Waglerin-2 and 3 are isolated from the same snake species1.

    Waglerin-1 selectively blocks the nAChRε subunit with an IC50 of 50 nM2 and is a potent blocker of muscle nAChR3. Waglerin-1 is also a modulator of GABA(A) receptors with potentiating and suppressing effects. Its IC50 and EC50 value is 24 μM4.

    Target nAChR, GABA(A) receptors
    Peptide Content: 100%
    Lyophilized Powder
    Last update: 02/01/2022

    Waglerin-1 (#STW-001) is a highly pure, synthetic, and biologically active peptide toxin.

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    For research purposes only, not for human use
    Shipping and Ordering information

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