Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002) is the original antigen used for immunization during Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002) generation. The blocking peptide binds and ‘blocks’ Anti-Bestrophin-2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat colon membranes:
- Western blot analysis of human colon cancer cell lines Colo-205 (lanes 1 and 4), human T-84 colonic adenocarcinoma cell line (lanes 2 and 5) and human colon cancer HT-29 cell line (lanes 3 and 6):
- Expression of Bestrophin-2 in rat colonImmunohistochemical staining of rat colon paraffin embedded sections using Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:100), (brown). BEST2 is expressed in the columnar absorptive cells of the colonic epithelium (arrows). Hematoxilin is used as the counterstain.
- (C)DPAQGYKDHTLD, corresponding to amino acid residues 259-270 of mouse Bestrophin-2 (Accession Q8BGM5).