Overview
Cat #: BLP-BC002
Type: Synthetic peptide
Form: Lyophilized powder
Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002) is the original antigen used for immunization during Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002) generation. The blocking peptide binds and ‘blocks’ Anti-Bestrophin-2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat colon membranes:1. Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:200).
2. Anti-Bestrophin-2 (extracellular) Antibody, preincubated with Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002). - Western blot analysis of human colon cancer cell lines Colo-205 (lanes 1 and 4), human T-84 colonic adenocarcinoma cell line (lanes 2 and 5) and human colon cancer HT-29 cell line (lanes 3 and 6):1-3. Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:200).
4-6. Anti-Bestrophin-2 (extracellular) Antibody, preincubated with Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002). - Expression of Bestrophin-2 in rat colonImmunohistochemical staining of rat colon paraffin embedded sections using Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:100), (brown). BEST2 is expressed in the columnar absorptive cells of the colonic epithelium (arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
Accession (Uniprot) Number Q8BGM5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.