CACNA1A/Cav2.1 Blocking Peptide (#BLP-CC001) is the original antigen used for immunization during Anti-CACNA1A (CaV2.1) Antibody (#ACC-001) generation. The blocking peptide binds and ‘blocks’ Anti-CACNA1A/Cav2.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of CACNA1A in mouse cerebellumImmunohistochemical staining of mouse cerebellum with Anti-CACNA1A (CaV2.1) Antibody (#ACC-001), (1:100). A. CACNA1A channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (vertical arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein in the section demonstrates the location of astrocytic fibers in the molecular layer. C. Merged image of panels A and B.
- (C)PSSPERAPGREGPYGRE, corresponding to amino acid residues 865-881 of rat CACNA1A (Accession P54282).