CACNG4 Blocking Peptide (#BLP-CC114) is the original antigen used for immunization during Anti-CACNG4 Antibody (#ACC-114) generation. The blocking peptide binds and ‘blocks’ Anti-CACNG4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human SH-SY5Y brain neuroblastoma (lanes 3 and 7) and rat PC12 pheochromocytoma adrenal gland (lanes 4 and 8) lysates:1-4. Anti-CACNG4 Antibody (#ACC-114), (1:800).
5-8. Anti-CACNG4 Antibody, preincubated with CACNG4 Blocking Peptide (#BLP-CC114).
- (C)EKNKELRFKTKRE, corresponding to amino acid residues 208-220 of rat CACNG4 (Accession Q8VHW9).